Life Technologies Corporation

David Bourdon

Paul Nelson

Niroshan Ramachandran

Gengxin Chen

Philip Martin

Lihao Meng

Suzy Van Le

Niroshan Ramchandran

A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. A lysis solution is passed through the filter in a second flow direction opposite the first direction, thereby dislodging the plurality of cells captured on the filter, and resuspending the plurality of cells in the lysis solution on the first side of the filter. The lysis solution lyses the plurality of cells to produce a cellular lysate. The cellular lysate is passed through the filter in the first flow direction.

Filter based system for capture of lysis of suspended cells

The disclosure provides SLE biomarkers. The disclosure further provides kits and methods of diagnosing, prognosing, and stratifying subjects with the disease by utilizing SLE biomarkers.

Biomarkers for systemic lupus erythematosus

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Carlsbad, CA, United States of America

David Bourdon