Mycartis N.v.

David Bernasconi

Didier Falconnet

Mathieu Gaillard

José Gil

David Rebetez

A method for determining fluorescence values {&#x3c6;}of a set of I fluorescent microparticles {&#x3bc;P}of a multiplexed analysis, the microparticles being in a monolayer arrangement, includes acquiring a digital fluorescence image of the set of fluorescent microparticles {&#x3bc;P}; and computing, for each fluorescent microparticle &#x3bc;Pin the set of fluorescent microparticles {&#x3bc;P}, a fluorescence value &#x3c6;based only on pixels of the acquired image corresponding to said fluorescent microparticle &#x3bc;P. The method includes computing the fluorescence value &#x3c6;of the fluorescent microparticle &#x3bc;Pby correcting its first fluorescence &#x3c6;by a cross-talk fluorescence contribution &#x3c6;in the first fluorescence &#x3c6;from other fluorescent microparticles {&#x3bc;P}in the set of fluorescent microparticles {&#x3bc;P}.

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La Neuveville, Switzerland

David Bernasconi