Illumina Cambridge Limited

Solexa, Inc.

David Bentley

Roberto Rigatti

Niall Anthony Gormley

Geoffrey Paul Smith

Shujun Luo

Jonathan Mark Boutell

Colin Lloyd Barnes

Tobias William Barr Ost

Vincent Peter Smith

Eric Hans Vermaas

Graham John Worsley

Shankar Balasubramanian

David Klenerman

The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5' ends and at their 3′ ends.

Method of preparing libraries of template polynucleotides

The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5' ends and at their 3&#x2032; ends.

The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.

Method for sequencing a polynucleotide template

Method for sequencing a polynucelotide template

A device comprising an array of molecules immobilised on a solid surface is disclosed, wherein the array has a surface density which allows each molecule to be individually resolved, e.g. by optical microscopy. Therefore, the arrays of the present invention consist of single molecules are more spatially distinct than the arrays of the prior art.

Arrayed biomolecules and their use in sequencing

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Nr Saffron Walden, United Kingdom

David Bentley