Harvard College

Massachusetts Eye and Ear Infirmary

University of Illinois

The Brigham and Women's Hospital, Inc.

David B Kolesky

Jennifer Ann Lewis

Mark Andrew Skylar-Scott

Kimberly A Homan

Ryan L Truby

Robert J Wood

Joseph Thomas Muth

Michael J McKenna

John Rosowski

Daniel M Vogt

Yigit Menguc

Aaron K Remenschneider

Daniel J Lee

Katharina Theresa Kroll

Nicole Black

Alexander D Valentine

George M Church

Christopher M Hansen

John J Vericella

Patrick Lustenberger

Jochen Mueller

Elliott Kozin

Sebastien G M Uzel

Joseph Bonventre

Amelia Sydney Gladman

Steven Kranz

Elliot Kozin

Willie Wu

Alex H M Ng

Yen-Chih Lin

Navin R Gupta

Michael T Valerius

Ryuji Morizane

Michael J Mckenna

Sebastien GM Uzel

Mark A Scott

Described are methods of enhancing development of renal organoids, methods of using the same, and kits.

Methods of enhancing development of renal organoids and methods of using the same

Described are methods for producing multi-layered tubular tissue structures, tissue structures produced by the methods, and their use.

Methods of producing multi-layered tubular tissue constructs

This disclosure features artificial tympanic membrane graft devices and two-component bilayer graft devices that include a scaffold having a plurality of ribs made of a first material and a plurality of spaces between the ribs filled or made with the first material, a different, second material, a combination of the first and a second materials, or a combination of a second material and one or more other different materials. The bilayer graft devices have two components or layers. One component, e.g., the underlay graft device, can include a projection, and the second component, e.g., the overlay graft device, can include an opening that corresponds to the projection (or vice versa) so that the opening and the projection can secure the two layers together in a 'lock and key' manner. This disclosure also features methods of making, using, and implanting the three-dimensional artificial tympanic membrane and bilayer graft devices.

Artificial tympanic membrane devices and uses

Described are devices and methods for use in connection with organ replacement or organ assist therapy in a patient.

Living devices for replacement of organs or specific organ functions, methods and uses of the same

Methods of tissue engineering, and more particularly methods and compositions for generating various vascularized 3D tissues, such as 3D vascularized embryoid bodies and organoids are described. Certain embodiments relate to a method of generating functional human tissue, the method comprising embedding an embryoid body or organoid in a tissue construct comprising a first vascular network and a second vascular network, each vascular network comprising one or more interconnected vascular channels; exposing the embryoid body or organoid to one or more biological agents, a biological agent gradient, a pressure, and/or an oxygen tension gradient, thereby inducing angiogenesis of capillary vessels to and/or from the embryoid body or organoid; and vascularizing the embryoid body or organoid, the capillary vessels connecting the first vascular network to the second vascular network, thereby creating a single vascular network and a perfusable tissue structure.

Methods of generating functional human tissue

A present disclosure relates to a system for automatic design and manufacturing of 3D printing units and 3D products. The system is configured to obtain specification of a target structure corresponding to a 3D product; automatically determine a design and a printing path of a 3D printing unit based on the specification of the target structure, wherein the 3D printing unit is a micronozzle unit configured to print the target structure of the 3D product; automatically determine a manufacturing procedure to print the 3D printing unit based on the specification of the target structure; and instruct a 3D printer to print the 3D printing unit according to the manufacturing procedure and the printing path of the 3D printing unit.

Systems and methods for automated nozzle design and 3D printing

A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.

Tubular tissue construct and a method of printing

A printed stretchable strain sensor comprises a seamless elastomeric body and a strain-sensitive conductive structure embedded in the seamless elastomeric body. The strain-sensitive conductive structure comprises one or more conductive filaments arranged in a continuous pattern. A method of printing a stretchable strain sensor comprises depositing one or more conductive filaments in a predetermined continuous pattern into or onto a support matrix. After the depositing, the support matrix is cured to embed a strain-sensitive conductive structure in a seamless elastomeric body.

Printed stretchable strain sensor

A printed tissue construct comprises one or more tissue patterns, where each tissue pattern comprises a plurality of viable cells of one or more predetermined cell types. A network of vascular channels interpenetrates the one or more tissue patterns. An extracellular matrix composition at least partially surrounds the one or more tissue patterns and the network of vascular channels. A method of printing a tissue construct with embedded vasculature comprises depositing one or more cell-laden filaments, each comprising a plurality of viable cells, on a substrate to form one or more tissue patterns. Each of the one or more tissue patterns comprises one or more predetermined cell types. One or more sacrificial filaments, each comprising a fugitive ink, are deposited on the substrate to form a vascular pattern interpenetrating the one or more tissue patterns. The vascular pattern and the one or more tissue patterns are at least partially surrounded with an extracellular matrix composition. The fugitive ink is then removed to create vascular channels in the extracellular matrix composition, thereby forming an interpenetrating vascular network in a tissue construct.

Method of printing a tissue construct with embedded vasculature

A multinozzle deposition system for direct write applications comprises a body including a first network of microchannels embedded therein, where the first network of microchannels extends from a parent microchannel through a series of furcations to a plurality of branching microchannels. The series consists of k generations with furcation number m where the kgeneration includes mbranching microchannels. A first end of the body includes a single inlet to the parent microchannel and a second end of the body includes moutlets from the branching microchannels, where k is an integer greater than or equal to 1 and m is an integer greater than or equal to 2. The body comprises a material having a sufficient rigidity to sustain a pressure in the microchannels of about 690 kPa or greater without distortion.

Multinozzle deposition system for direct write applications

Growing community of inventors

Cambridge, MA, United States of America

David B Kolesky