Eppendorf-Netheler-Hinz Gmbh

Brinkman Instruments, Inc.

Attainment Company

David Alan Uhen

Troy A VerStrate

Don Bastian

Electronic tablet case

An apparatus for electroporation stores a level of charge, specified by a user, on a capacitor, which is delivered to a cuvette through an optically isolated high voltage switch. The capacitor is charged through a charging system, including a current mode pulse width modulation control circuit, which monitors the current in the primary winding of a transformer and supplies a pulse width modulated signal, limiting the current on every pulse to a level set by the microcontroller, to the controlling transistor in order to generate the drive to the primary winding of the transformer. A controlled amount of energy is transferred through each pulse to the capacitor. The microcontroller monitors the voltage on the capacitor up to a threshold level to predict the number of pulses necessary to store the requested amount of charge on the capacitor. The microcontroller will then count the number of pulses until the number of pulses necessary to store the requested amount of charge on the capacitor has been reached. At this point the requested voltage is delivered to the cuvette through the optically isolated high voltage switch comprised of sensitive gate SCRs coupled together in series. The voltage and current at the cuvette are monitored and input to the microcontroller so that the impedance at the cuvette is calculated by the microcontroller and errors in the composition of the solution within the cuvette are detected.

Apparatus for electroporation

A modular chromatography column heating and temperature control system includes a heater base module with a compartment for the chromatography column that includes a heater plate. A set of heater blocks is provided in pairs, each heater block having opposite main faces in which are formed channels that match chromatography columns of different diameters. The heater blocks are mounted in pairs on the heater plate with the chromatography column held between them in matching channels on the two adjacent main faces of the heater blocks. An appropriate number of pairs of heater blocks are mounted to the heater plate so that the pairs of heater blocks cover as much of the length of the chromatography column as desired. The set of available pairs of heater blocks may include heater blocks of different lengths, so that appropriate selections of pairs of heater blocks can be made to best match the length of the chromatography column. The heater blocks are formed of a good heat conductor, such as aluminum, to efficiently conduct heat from the heater plate to the chromatography column or away from the chromatography column to maintain the column at a desired temperature.

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Burlington, WI, United States of America

David Alan Uhen