The United States of America As Represented by the Administrator of the

United States of America As Represented by the Administrator of Nasa

The United States of America As Represented by the Administrator

David A Wolf

Thomas John Goodwin

Ray P Schwarz

Glenn F Spaulding

Tacey L Prewett

Tinh T Trinh

Alvin T Rockhill

William H Beeson

Clarence F Sams

John H Cross

Marian L Lewis

Mary H Huls

Tammy Wolf

A highly effective technique for fertilizing an oocyte with a sperm cell to produce a zygote can be carried out in a bioreactor and the zygote can optionally be cultured in the bioreactor into a blastocyst stage embryo for implantation into the uterus of a female recipient. The success rate and probability of fertilization is enhanced by operating the bioreactor reactor under conditions of inter-galactic motion which increases the probability of the sperm coming in close proximity to and fertilizing the oocyte. This method involves the steps of collecting an oocyte from a female donor, collecting sperm from a male donor, fertilizing the oocyte with the sperm in a rotating bioreactor to produce a zygote, culturing the zygote into a blastocyst stage embryo, and implanting the blastocyst stage embryo into a uterus of a recipient female.

Method for in vitro fertilization in a bioreactor

The present invention provides systems for growing two or three dimensional mammalian cells within a culture medium facilitated by an electromagnetic field, and preferably, a time varying electromagnetic field. The cells and culture medium are contained within a fixed or rotating culture vessel, and the electromagnetic field is emitted from at least one electrode. In one embodiment, the electrode is spaced from the vessel. The invention further provides methods to promote neural tissue regeneration by means of culturing the neural cells in the claimed system. In one embodiment, neuronal cells are grown within longitudinally extending tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time varying electrical current is conducted, the conductive channels being positioned within a culture medium.

Growth stimulation of biological cells and tissue by electromagnetic fields and uses thereof

A process for propagating a pathogen in a three-dimensional tissue mass cultured at microgravity conditions in a culture vessel containing culture media and a culture matrix is provided. The three-dimensional tissue mass is inoculated with a pathogen and pathogen replication in the cells of the tissue mass achieved.

Pathogen propagation in cultured three-dimensional tissue mass

Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under micro-gravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated micro-gravity created in a horizontal rotating wall culture vessel. The medium used for culturing the cells, especially a mixture of epithelial and mesenchymal cells contains a mixture of Mem-alpha and Leibovits L15 supplemented with glucose, galactose and fructose.

Production of normal mammalian organ culture using a medium containing

Artificial high-fidelity three-dimensional human urogenital tract carcinomas are propagated under in vitro-microgravity conditions from carcinoma cells. Artificial high-fidelity three-dimensional human urogenital tract carcinomas are also propagated from a coculture of normal urogenital tract cells inoculated with carcinoma cells. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.

Cultured high-fidelity three-dimensional human urogenital tract

The process of the present invention relates to a three dimensional co-culture process.

Three-dimensional co-culture process

Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under microgravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.

Method for producing non-neoplastic, three dimensional, mammalian tissue

The present invention relates to a multicellular, three-dimensional, living mammalian tissue. The tissue is produced by a co-culture process wherein two distinct types of mammalian cells are co-cultured in a rotating bioreactor which is completely filled with culture media and cell attachment substrates. As the size of the tissue assemblies formed on the attachment substrates changes, the rotation of the bioreactor is adjusted accordingly.

Multi-cellular, three-dimensional living mammalian tissue

A bio-reactor system wherein a tubular housing contains an internal circularly disposed set of blade members and a central tubular filter all mounted for rotation about a common horizontal axis and each having independent rotational support and rotational drive mechanisms. The housing, blade members and filter preferably are driven at a constant slow speed for placing a fluid culture medium with discrete microbeads and cell cultures in a discrete spatial suspension in the housing. Replacement fluid medium is symmetrically input and fluid medium is symmetrically output from the housing where the input and the output are part of a loop providing a constant or intermittent flow of fluid medium in a closed loop.

Method for culturing mammalian cells in a perfused bioreactor

The present invention relates to a 3-dimensional cell to tissue and maintenance process, more particularly to methods of culturing cells in a culture environment, either in space or in a gravity field, with minimum fluid shear stress, freedom for 3-dimensional spatial orientation of the suspended particles and localization of particles with differing or similar sedimentation properties in a similar spatial region.

Three-dimensional cell to tissue assembly process

A suspension cell culture system where a culture chamber is rotatable about a horizontal axis and has a vertical large area oxygen transmissible membrane spaced a distance about 0.25 inches less than 1.0 inches from a facing vertical wall surface for effective transmission of oxygen to cells in suspension in the culture chamber. The facing vertical wall surface can be a dialysis membrane for exchange of fresh nutrient from a dialysis chamber with cell waste product in the culture chamber.

High aspect reactor vessel and method of use

A bio-reactor system where cell growth microcarrier beads are suspended in a zero head space fluid medium by rotation about a horizontal axis and where the fluid is continuously oxygenated from a tubular membrane which rotates on a shaft together with rotation of the culture vessel. The oxygen is continuously throughput through the membrane and disbursed into the fluid medium along the length of the membrane.

Method for culturing mammalian cells in a horizontally rotated bioreactor

The process of the present invention relates to a three dimensional co-culture process. Wherein two distinct types of mammalian cells are co-cultured in a rotating bioreactor which is completely filled with culture media and cell attachment substrates. As the size of the tissue assemblies formed on the attachment substrates changes the rotation of the bioreactor is adjusted accordingly.

Growing community of inventors

Houston, TX, United States of America

David A Wolf