USA As Represented by Secretary of the Navy

Geo-centers, Inc.

David A Stenger

Baochuan Lin

Anthony P Malanoski

Joel M Schnur

Scott A Trammell

Daniel Zabetakis

Walter J Dressick

Jasenka Verbarg

Brett D Martin

Jacque H Georger, Jr

Paul T Charles

Thomas L Fare

Charles H Patterson, Jr

Mark S Spector

Marie J Archer

Zheng Wang

Frances S Ligler

Chih-Chang Chu

David Kurt Gaskill

Alan S Rudolph

Rachael L Myers-Ward

Scott G Walton

Brandy J White

Barry J Spargo

Jacque Georger

James P Whelan

Sandra C Hangarter

Clark J Tibbetts

Jonathan D Yuen

Gary J Vora

Kate M Blaney

Joyce C Breger

Jennifer Thornton

Jeff Erickson

Elizabeth A Walter

Dzung C Thach

Jennifer Johnson

Donald Seto

Robb K Rowley

Russell P Kruzelock

Eric H Hanson

Brian K Agan

Jeffrey S Erickson

Described herein are ultra-thin nanocellulose flexible electronic device on which SU-8, an epoxy material which can become highly stressed upon UV exposure, is printed on desired areas. Upon UV exposure and then release from the surface it is anchored on, the nanocellulose device will spontaneously self-mold into a desired form due to stress differences between the SU-8 and the nanocellulose sheet. The flexible electronics can be manufactured using standard printed circuit board processing techniques, including electroless metallization and soldering of surface mount components.

Four dimensional printed circuit boards

An electrochemical cell includes a working electrode in contact with an aqueous electrolyte solution, a counter electrode in contact with the aqueous electrolyte solution, and a reference electrode in contact with the aqueous electrolyte solution. The working electrode comprises a plasma modified epitaxial synthesized graphene surface fabricated on SiC.

Plasma modified epitaxial fabricated graphene on SiC for electrochemical trace detection of explosives

Systems and methods are provided for identification of a detection event relevant in applications focused on real-time or near real-time reporting in a continuous monitoring application. A controller collects data from a plurality of sensors and compares recent data to background data to determine whether an event has occurred. A system in accordance with an embodiment of the present disclosure is considered to have detected an event of interest when at least a specified minimum number of sensors report a detection event at the same time.

Analyzing reflectance based color changes in sensing applications

Described herein is an approach using inexpensive, disposable chemical sensor probes that can be mounted on a small unmanned aerial vehicles (UAVs) and used to analyze a site (such as one known or suspected to contain explosive residue, spilled material or contaminated soil) without the need for a person to conduct ground operations at the site. The method involves contacting a soil or a surface with a filter paper wetted with a solvent, then subjecting the filter paper to spectroscopy, thus detecting a possible variation indicative of one or more analytes, wherein the solvent is a deep eutectic solvents consisting of a mixture of ethylene glycol and choline chloride.

Spectroscopic method for the extraction and electrochemical detection of explosives and explosive components in soils using filter paper, and electrolyte

Described herein is an approach using inexpensive, disposable chemical sensor probes that can be mounted on a small unmanned aerial vehicles (UAVs) and used to analyze a site (such as one known or suspected to contain explosive residue, spilled material or contaminated soil) without the need for a person to conduct ground operations at the site. The method involves contacting a soil or a surface with a filter paper wetted with a solvent, then subjecting the filter paper to voltammetry and/or spectroscopy, thus detecting a possible variation indicative of one or more analytes, wherein the solvent is the deep eutectic solvent consisting of a mixture of ethylene glycol and choline chloride.

Unmanned aerial vehicle system for the extraction and electrochemical detection of explosives and explosive components in soils using filter paper and electrolyte

Described herein is an approach using inexpensive, disposable chemical sensor probes that can be mounted on a small unmanned aerial vehicles (UAVs) and used to analyze a site (such as one known or suspected to contain explosive residue, spilled material or contaminated soil) without the need for a person to conduct ground operations at the site. The method involves contacting a soil or a surface with a filter paper wetted with a solvent, then subjecting the filter paper to voltammetry and/or spectroscopy, thus detecting a possible variation indicative of one or more analytes, wherein the solvent is selected from the group consisting of polar aprotic or protic solvents having a boiling point of at least 100&#xb0; C., room temperature ionic liquids, and deep eutectic solvents.

Method for the extraction and electrochemical detection of explosives and explosive components in soils using electrodes, filter paper, and electrolyte

The present invention relates to pathogen detection and identification by use of DNA resequencing microarrays. The present invention also provides resequencing microarray chips for differential diagnosis and serotyping of pathogens present in a biological sample. The present invention further provides methods of detecting the presence and identity of pathogens present in a biological sample.

Re-sequencing pathogen microarray

A method of: providing a solid surface having a dendrimer molecule bound thereto and a single-stranded probe nucleic acid immobilized to the dendrimer; contacting the solid surface with a sample suspected or known to contain a double-stranded complimentary target nucleic acid; denaturing the target nucleic acids at thermal conditions and in a salt concentration sufficient to denature the target nucleic acids to produce denatured nucleic acids; and cooling the sample to allow hybridization of the denatured nucleic acids to the probe nucleic acids. An article having: one or more paramagnetic microbeads; a dendrimer molecule bound to the beads; and a probe nucleic acid immobilized to the dendrimer.

Solid phase for capture of nucleic acids

An apparatus having within or as part of a housing; a sample port; a microarray port; a lysis module; a purification module for containing a solid phase for binding of oligonucleotides; a thermocycling module for containing a polymerase chain reaction; a fragmentation module; and a microarray module for holding a microarray and a liquid in contact with the microarray. The apparatus is configured to be coupled to a device for: pumping a liquid through, in order, the lysis, purification, thermocycling, fragmentation, and microarray modules; sonicating any contents of the lysis module; thermocycling the thermocycling module to perform the polymerase chain reaction; heating the fragmentation module to fragment any oligonucleotides contained therein; circulating a fluid over the surface of the microarray; and performing one or more washing or staining steps on the microarray.

Automated sample-to-microarray apparatus and method

A computer-implemented biological sequence identifier (CIBSI) system and method for selecting a subsequence from biological sequence data according to at least one selection parameter. The at least one selection parameter corresponds to a likelihood of returning a meaningful result from a similarity search.

Computer-implemented method, computer readable storage medium, and apparatus for identification of a biological sequence

A method of: submitting reference sequences to a taxonomic database to produce taxonomic results; and reporting a taxonomic identification based on the taxonomic results. The reference sequences are the output of genetic database queries that return a score for each reference sequence. A method for processing a biological sequence obtained from an assay by: converting base calls located in a predetermined list of positions within the biological sequence to N; and determining the ratio of single nucleotide polymorphisms in the biological sequence relative to a reference sequence. Each entry in the predetermined list of positions represents the capability of a substance hybridizing to a microarray used to generate the biological sequence. The substance is not the nucleic acid of a target pathogen.

Computer-implemented biological sequence identifier system and method

The use of sugar-containing hydrogels as very highly porous, aqueous support material for the immobilization of oligonucleotides, peptides, proteins, antigens, antibodies, polysaccharides, and other biomolecules for sensor applications. Unusually large sizes of interconnected pores allow large target molecules to pass rapidly into and through the gel and bind to immobilized biomolecules. Sugar-containing hydrogels have extremely low non-specific absorption of labeled target molecules, providing low background levels. Some hydrogel materials do not have this type of homogeneous interconnected macroporosity, thus large target molecules cannot readily diffuse through them. Additionally, they nearly always experience non-specific absorption of labeled target molecules, limiting their usefulness in sensor applications. A method is provided for preparing sugar polyacrylate hydrogels with functional chemical groups which covalently bond oligonucleotides and peptides. A method for copolymerizing acrylate-terminated oligonucleotides with sugar acrylate monomers and diacrylate cross-linking agents is also provided.

Immobilization of oligonucleotides and proteins in sugar-containing hydrogels

Sugar-containing hydrogel for immobilization

A PCR method involving: providing a biological sample suspected of containing one or more pathogen nucleic acids; adding a plurality of PCR primers corresponding to genes found in the pathogens; and performing a polymerase chain reaction on the sample to amplify a subset of the nucleic acids that correspond to the genes. The primers include at least one primer pair for each pathogen, and the primers contain a tail sequence that is not homologous any pathogen DNA or to any background DNA in the sample. The concentration of at least one primer in the polymerase chain reaction is no more than about 100 nM.

Multiplexed polymerase chain reaction for genetic sequence analysis

A computer-implemented method as follows. Providing a list of target sequences associated with one or more organisms. Providing a list of candidate prototype sequences suspected of hybridizing to one or more of the target sequences. Generating a collection of probes corresponding to each candidate prototype sequence, each collection of probes having a set of probes for every subsequence. The sets consist of the corresponding subsequence and every variation of the corresponding subsequence formed by varying a center nucleotide of the corresponding subsequence. Generating a set of fragments corresponding to each target sequence. Calculating the binding free energy of each fragment with a perfect complimentary sequence of the fragment. Determining which extended fragments are perfect matches to any of the probes. Assembling a base call sequence corresponding to each candidate prototype sequence.

Design and selection of genetic targets for sequence resolved organism detection and identification

Growing community of inventors

Herndon, VA, United States of America

David A Stenger