University of Minnesota

The United States of America

Other

The United States of America As Represented by the Secretary of

Basf Plant Science Gmbh

David A Somers

Burle G Gengenbach

Donald L Wyse

John W Gronwald

Kimberly A Torbert

Margaret A Egli

Sheila M Lutz

William B Parker

Iris Tzafrir

Neil E Olszewski

Benham Lockhart

Howard W Rines

Friedrich Srienc

Paula Olhoft

Olin D Anderson

Ann E Blechl

Lorelei C Marshall

John K Jackson

J J Hahn

Arthur C Eschenlauer

Juantia M Sellner, Deceased

Jonathan M Shaver

Douglas C Bittel

Kevin L Van Dee

Shelia M Lutz

The invention provides a method to enhance-mediated transformation of plant cells, parts and tissues, thereby enhancing the production of transgenic plants.

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The invention provides a method to enhance Agrobacterium-mediated transformation of plant cells, parts and tissues, thereby enhancing the production of transgenic plants.

Method to enhance agrobacterium-mediated transformation of plants

An isolated and purified DNA molecule comprising a sugarcane bacilliform virus promoter and expression cassettes comprising said promoter are provided. Also provided is a method of using a sugarcane bacilliform virus promoter to express proteins, RNA transcripts, or mixtures thereof, in transgenic plants.

Sugarcane bacilliform virus promoter

The present invention provides methods for preparing herbicide tolerant corn plants. Also provided are herbicide tolerant corn plants, as well as seeds and progeny derived from these plants.

Gene combinations for herbicide tolerance in corn

The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells.

Methods and a maize acetyl CoA carboxylase gene for altering the oil content of plants

The present invention provides a complete cDNA sequence and partial DNA sequences encoding maize acetyl CoA carboxylase and methods for altering the oil content of plants by introducing and expressing a maize acetyl CoA carboxylase gene in plant cells.

Transgenic plants expressing maize acetyl COA carboxylase gene and method of altering oil content

The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and a method introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase crystallized enzyme.

Methods for expressing a maize acetyl CoA carboxylase gene in host cells

A genetically engineered Pseudomonas oleovorans phaC1 polyhydroxyalkanoate (PHA) polymerase having tailored substrate specificity is provided. The modified PHA polymerase is preferably a 'bispecific' PHA polymerase capable of copolymerizing a short chain length monomer and a medium chain length monomer is provided. Methods for making the modified PHA polymerase and for making nucleic acids encoding the modified PHA polymerase are also disclosed, as are methods of producing PHA using the modified PHA polymerase. The invention further includes methods to assay for altered substrate specificity.

Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific

Novel transgenic plants and plant cells are capable of biosynthesis of polyhydroxyalkanoate (PHA). Heterologous enzymes involved in PHA biosynthesis, particularly PHA polymerase, are targeted to the peroxisome of a transgenic plant. Transgenic plant materials that biosynthesize short chain length monomer PHAs in the absence of heterologous .beta.-ketothiolase and acetoacetyl-CoA reductase are also disclosed.

Polyhydroxyalkanoate synthesis in plants

The present invention provides the complete cDNA sequence of maize acetyl CoA carboxylase and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. The method of imparting herbicide tolerance to a plant includes the steps of introducing an expression cassette encoding a plant acetyl CoA carboxylase or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene operably linked to a promoter functional in plant cells, into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell substantially tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into other host cells to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme.

Methods and an acetyl CoA carboxylase gene for conferring herbicide

The present invention is directed to methods of improving flour by altering glutenin content in seeds of wheat and non-wheat plants. The invention relates to methods of introducing a recombinant construct comprising a glutenin gene into a parental plant.

Glutenin genes and their uses

A method to prepare fertile transgenic oat plants by transforming cells of, or derived from, mature embryos is provided, as well as plants prepared by said method.

Growing community of inventors

Roseville, MN, United States of America

David A Somers