University of California

The United States Government As Represented by the Department of Veterans Affairs

Cornell Research Foundation Inc.

Fundacao Oswaldo Cruz-fiocruz

Other

Microbedx, Inc.

David A Haake

Lee W Riley

Isadora Cristina Siqueira

James Matsunaga

Julio Henrique Rosa Croda

Albert I Ko

Tracy Ann Young

Mitermayer Galvão Reis

Michele Barocchi

Cheryl I Champion

David R Blanco

Michael A Lovett

James N Miller

Michele Anne Barocchi

Bernard M Churchill

Ellen S Shang

Yang Li

Joseph Chihping Liao

Marc A Suchard

Mitra Mastali

Colin Halford

Jane T Babbitt

Mitermayer Galvào Reis

Daniel Gussin

Gabriel K Monti

Colin W Halford

Described are probes and methods for detecting antibiotic susceptibility of a specimen. The method comprises contacting the specimen with an oligonucleotide probe that specifically hybridizes with a target nucleic acid sequence region of ribosomal RNA. The target sequence is at the junction between a pre-ribosomal RNA tail and mature ribosomal RNA of 23S or 16S rRNA. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic susceptibility.

Antibiotic susceptibility testing using probes for preribosomal RNA

The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in thesp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection withspecies that are capable of producing disease in humans and other mammals, including those of veterinary importance.

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Proteins with repetitive bacterial-Ig-like (Big) domains present in leptospira species

Described are probes and methods for detecting pathogens and antibiotic resistance of a specimen. The method comprises contacting the specimen with a growth medium; and lysing the specimen to release nucleic acid molecules from the specimen. The lysate of the specimen is contacted with a capture probe immobilized on a substrate, wherein the capture probe comprises an oligonucleotide that specifically hybridizes with a first target nucleic acid sequence region of ribosomal RNA. The lysate is in contact with a detector probe that comprises a detectably labeled oligonucleotide that specifically hybridizes with a second target nucleic acid sequence region of ribosomal RNA. The presence or absence of labeled oligonucleotide complexed with the substrate is determined. Detection of labeled oligonucleotide complexed with the substrate is indicative of the presence of pathogen. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic resistance.

The present invention presents novel leptospiral membrane lipoproteins, LipL1 and LipL2, associated with pathogenic strains of Leptospira. LipL1 is of about 35 kDa, and LipL2 is of about 41 kDa. Also disclosed are the method for purifying these proteins from Leptospira, their nucleotide and amino acid sequences, the cloning of the genes encoding the proteins and their recombinant proteins, methods for producing antibodies to these proteins, the resulting antibodies. These proteins, their immunogenic fragments, and antibodies against them, are useful for inducing an immune response to pathogenic Leptospira as well as providing a diagnostic target for leptospirosis.

Leptospira rare outer membrane proteins

An antigenic preparation is provided which contains a 31 Kd outer membrane protein from Leptospira which can be used immunologically as a vaccine for leptospirosis caused by this organism.

Cloned Leptospira outer membrane protein

Growing community of inventors

Los Angeles, CA, United States of America

David A Haake