Scarab Genomics, LLC

Wisconsin Alumni Research Foundation

David A Frisch

Frederick R Blattner

Gyorgy Posfai

Robert E Novy

Charles Landry

Eric A Steffen

Hyunsic Choi

John Brandon

Guy Plunkett

John Walter Campbell

Terrance M Henker

Christopher R Blattner

Jose A Sanchez

Cristopher R Blattner

Charles F Landry

Provided herein arehost strains with improved capacity for producing recombinant proteins.

Reduced genomelacking toxin-antitoxin genes

Provided herein are methods for the improved production of periplasmic-targeted recombinant proteins inhost strains. Also provided arehost strains with improved capacity for producing recombinant proteins.

Methods and compositions for improved expression of recombinant proteins

The present invention relates to the use of redox agents for purification of the CRM 197 variant of diphtheria toxin. The invention further relates to multi-step purification of CRM 197 from bacterial fermentates.

Purification of CRM 197 from bacteria

Rediced genome or native K12 strainbacteria comprising expression vectors encoding a recombinant CRM197 protein and their use in the production of CRM 197 is provided. The CRM 197 protein may be fused to a signal sequence that directs the expressed CRM197 protein to the periplasm of thehost.

Enhanced production of recombinant CRM197 in

bacteria comprising genetic modifications to enhance fermentability and production of protein and nucleic acids are provided.

Bacteria with improved metabolic capacity

The present invention provides a bacterium having a genome that is genetically engineered to be smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.

Prophage element-free bacteria

A bacteria lacking genomic and non-genomic IS elements is provided. The bacteria may be more stable and useful for the production of amino acids, polypeptides, nucleic acids and other products.

Growing community of inventors

Fitchburg, WI, United States of America

David A Frisch