Massachusetts Institute of Technology

Whitehead Institute for Biomedical Research

David A Canner

Tyler E Jacks

Daniel Griffith Anderson

Hidde L Ploegh

Omar Khan

Jasdave S Chahal

Robert Samuel Langer

Aviv Regev

Rebecca H Herbst

Robert S Langer

Amy Li

Compositions and methods for modified dendrimer nanoparticle ('MDNP') delivery of therapeutic, prophylactic and/or diagnostic agent such as large repRNA molecules to the cells of a subject have been developed. MDNPs efficiently drive proliferation of antigen-specific T cells against intracellular antigen, and potentiate antigen-specific antibody responses. MDNPs can be multiplexed to deliver two or more different repRNAs to modify expression kinetics of encoded antigens and to simultaneous deliver repRNAs and mRNAs including the same UTR elements that promote expression of encoded antigens.

Compositions and methods for modified dendrimer nanoparticle delivery

The present invention discloses novel methods, compositions, and uses thereof for removing or overcoming immunosuppression. More specifically, the methods and compositions disclosed herein target effector Tcells by modulating ST2 and/or IL-33 signaling using pharmaceutical inhibitors and/or genetic ablation, whereby the levels and/or activities of effector Tcells in a tumor microenvironment are inhibited, and the infiltration of effector CD8cytotoxic T cells into tumor microenvironment increases. As a result, tumor growth is inhibited and tumor volume is reduced. The present invention also provides methods for identifying and isolating effector Tcells in a population of heterogeneous cells.

Methods and compositions for overcoming immunosuppression

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Cambridge, MA, United States of America

David A Canner