South Dakota State University

University of Minnesota

Boehringer Ingelheim Vetmedica Gmbh

Other

Boehringer Ingelheim Animal Health, Inc.

Boehringer Ingleheim Vetmedica, Inc.

David A Benfield

David E Gorcyca

Louis L Harris

Danny W Chladek

James E Collins

The invention includes kits for producing and using immunogenic compositions that include swine infertility and respiratory virus. The invention also includes vaccines and sera for treatment of Mystery Swine Disease (MSD), a method for producing the vaccine, methods for diagnosis of MSD, a viral agent that will mimic 'mystery swine disease' and antibodies to the viral agent useful in diagnosis and treatment of MSD. The serum contains mammalian antibodies which are effective in treating MSD.

Kits for detecting anti-SIRS antibodies

Immunogenic compositions that include SIRS virus

The invention includes a vaccine and sera for treatment of Mystery Swine Disease (MSD), a method for producing the vaccine, methods for diagnosis of MSD, a viral agent that will mimic 'mystery swine disease' and antibodies to the viral agent useful in diagnosis and treatment of MSD. The serum contains mammalian antibodies which are effective in treating MSD.

Kits for detecting swine infertility and respiratory syndrome (SIRS) virus

The invention includes a vaccine and sera for treatment of Mystery Swine Disease (MSD), a method for producing the vaccine, methods for diagnosis of MSD, a viral agent that will mimic &ldquo;mystery swine disease&rdquo; and antibodies to the viral agent useful in diagnosis and treatment of MSD. The serum contains mammalian antibodies which are effective in treating MSD.

Method for detecting swine infertility and respiratory virus

Immunogenic composition containing inactivated swine infertility and respiratory Syndrome virus

Vaccine for swine infertility and respiratory syndrome and method of use

Culture of swine infertility and respiratory syndrome virus in simian

The method of diagnosis of Mystery Swine Disease (MSD) is carried out with polyclonal or monoclonal antibody sera. Either the antibody sera or the biopsied tissue homogenate may be immobilized by contact by contact with a polystyrene surface or with a surface of another polymer for immobilizing protein. Preferably the monoclonal antibody is produced by a murine derived hybrid cell line wherein the antibody is an IgG or IgM type immunoglobulin. Example monoclonal antibodies to the infectious agent for SIRS are monoclonal antibody SDOW 12 and SDOW 17.

Method for diagnosis of mystery swine disease

Monoclonal antibody sera can be produced by hybridoma technique. After immunization of a mouse, pig, rat, rabbit or other appropriate species with Mystery Swine Disease (MSD) containing cell culture lysate, the spleen of the animal can be removed and converted into a whole cell preparation. Preferably the monoclonal antibody is produced by a murine derived hybrid cell line wherein the antibody is an IgG or IgM type immunoglobulin. Example monoclonal antibodies to the infectious agent for SIRS are monoclonal antibody SDOW 12 and SDOW 17.

Monoclonal antibodies to the Mystery Swine Disease virus

Growing community of inventors

Brookings, SD, United States of America

David A Benfield