Vineland Research and Innovations Centre Inc.

Daryl John Somers

Rumen Conev

Travis Wilfred Banks

The present invention provides methods for isolation of a member of a population which has one or more mutation(s) in one or more target sequence(s) in a population. The method may comprise the steps of: (a) pooling genomic DNA isolated from each member of the population in one or more dimensions; (b) amplifying the one or more target sequence(s) in the pooled genomic DNA, wherein optionally the amplification products are pooled; (c) sequencing the amplified products or obtaining the sequence reads for the amplified products, wherein, optionally, sequencing is by pair-end sequencing and further comprises merging the paired-end reads into composite read(s); (d) identifying the mutation(s) based on alignment-free sequence analysis of sequencing data, optionally by k-mer analysis and (e) identifying individual member(s) of the population comprising the one or more identified mutations in the target sequences, optionally by high-resolution DNA melting (HRM).

High throughput method of screening a population for members comprising mutation(s) in a target sequence using alignment-free sequence analysis

The invention is a new and distinct ornamental dwarf grapevine variety denominated 'VDG003'. The new grapevine carries the REN1 gene for resistance to powdery mildew and is characterized by, no visible hair on the young leaves, having relatively smooth leaf surface, having shallow and open lobe sinuses, and having small to medium yellow green berries.

Dwarf grapevine 'VDG003'

The invention is a new and distinct ornamental dwarf grapevine variety denominated 'VDG004'. The new grapevine carries the REN1 gene for resistance to powdery mildew and is characterized by light green leaves, no visible hair on the young leaves, not having prominent lobes on mature leaf, having smooth leaf surface, having greenish internodes with distinctive anthocyanin accumulation on the nodes, and having small light purple berries.

Dwarf grapevine 'VDG004'

The invention is a new and distinct ornamental dwarf grapevine variety denominated 'VDG001'. The new grapevine is characterized by light to medium green leaf color, reddish petiole, smooth mature leaf surface, petiole sinus with closed lobes, circular mature leaf, some veins with anthocyanin coloration and green berries.

Dwarf grapevine 'VDG001'

The invention is a new and distinct ornamental dwarf grapevine variety denominated 'VDG002'. The new grapevine is characterized by having approximately half of the mid-rib veins with medium red anthocyanin color, medium red anthocyanin color for petiole, medium red anthocyanin color for young shoots, almost flat mature leaf blades, lighter green color mature leaves, a lack of the characteristic downy white appearance of leaves in the female parent 'Pixie'&#x2122;, and yellow-green berries.

Growing community of inventors

Fenwick, Canada

Daryl John Somers