Dh Technologies Development Pte. Ltd.

Other

Darryl J Pappin

Subhasish Purkayastha

John Paul Wilson

Scott B Daniels

Nikita Khainovski

Michael Bartlet-Jones

Jonathan J Ipsaro

Subodh B Nimkar

Philip L Ross

Steven R Guertin

The invention relates to protease enzymes (that can be thermostable in some embodiments) with unique cleavage specificity as well as their production, isolation, activation and applications. These enzymes can be engineered for quick production and purification. The peptides produced by the action of these protease enzymes have unique properties for biochemical determination of protein sequence. In particular, using the steps of protease digestion, ionization of the specifically produced peptides and fragmentation of these peptides in a mass spectrometer, the amino acid sequence of the peptide may be read as a ladder from the N-terminus.

Proteases for the production of N-terminal argininyl- and lysinyl-peptides and methods of use in protein analysis

The present teachings provide methods for analyzing one or more amine-containing compounds in one or more samples using isobaric labels and parent-daughter ion transition monitoring (PDITM). In various embodiments, the methods comprise the steps of: (a) labeling one or more amine-containing compounds with different isobaric tags from a set of isobaric tags, each isobaric tag comprising a reporter ion portion; (b) combining at least a portion of each of the isobarically labeled amine-containing compounds to produce a combined sample; (c) subjecting at least a portion of the combined sample to PDITM; (d) measuring the ion signal of one or more of the transmitted reporter ions; and (e) determining the concentration of one or more of the isobarically labeled amine-containing compounds based at least on a comparison of the measured ion signal of the corresponding reporter ion to one or more measured ion signals of a standard compound.

Amine-containing compound analysis methods

Embodiments of the present invention relate to the determination of analyte characteristics based upon binding properties using mass analysis and differential labeling reagents.

Determination of analyte characteristics based upon binding properties

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Boxborough, MA, United States of America

Darryl J Pappin