Dh Technologies Development Pte. Ltd.

Applera Corporation

Dh Technologies Pte. Ltd.

Dh Technologies Ptd. Ltd.

Darryl J C Pappin

Sasi K Pillai

James M Coull

Subhasish Purkayastha

Subhakar Dey

Michael Bartlet-Jones

Nikita Khainovski

Michael Bartlett-Jones

Darryl D Spencer

Krishna G Upadhya

Xiongwei Yan

Joe Y Lam

Pau-Miau Yuan

Kuo-Liang Hsi

Sylvia W Yuen

Helena Huang

Sasi Pillai

Provided herein are labeling reagents for analyte determination in a sample, and methods of manufacturing the labeling reagents. By way of non-limiting example, analytes may include one or more of peptides, proteins, nucleic acid molecules, carbohydrates, lipids, steroids and/or small molecules. Also provided are labeled analytes themselves after one or more labeling reagents binds to an analyte. Analytes may be labeled for determination of the analyte by mass analysis, such as by mass spectrometry. Further provided are compounds that may be used in making the labeling reagents and labeled analytes, such as reporter group compounds. Also provided are kits that include at least one labeling reagent, and may further include or more other reagents, containers, enzymes, buffers, a labeled analyte (e.g., as a standard) and/or instructions. Further examples are also possible.

Labeling reagents for analyte determination and methods and compounds used in making the same

This invention pertains to methods, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.

Kits pertaining to analyte determination

In some embodiments, this invention pertains to active esters of N-substituted piperazine acetic acid, including isotopically enriched versions thereof. In some embodiments, this invention pertains to methods for the preparation of active esters of N-substituted piperazine acetic acid, including isotopically enriched versions thereof.

Active esters of N-substituted piperazine acetic acids, including isotopically enriched versions thereof

This invention pertains to methods, mixtures, kits and/or compositions for the determination of analytes by mass analysis using unique labeling reagents or sets of unique labeling reagents. The labeling reagents can be isomeric or isobaric and can be used to produce mixtures suitable for multiplex analysis of the labeled analytes.

Mass tags for quantitative analyses

This invention pertains to isobarically labeled analytes and fragment ions thereof.

Isobarically labeled analytes and fragment ions derived therefrom

Sets and compositions pertaining to analyte determination

In some embodiments, this invention pertains to isotopically enriched N-substituted piperazines. In some embodiments, this invention pertains to methods for the preparation of isotopically enriched N-substituted piperazines.

Isotopically enriched N-substituted piperazines and methods for the preparation thereof

Methods compositions and kits pertaining to analyte determination

Isobaric labels for mass spectrometric analysis of peptides and method thereof

In some embodiments, this invention pertains to isotopically enriched N-substituted piperazine acetic acids. In some embodiments, this invention pertains to methods for the preparation of isotopically enriched N-substituted piperazine acetic acids.

Isotopically enriched N-substituted piperazine acetic acids and methods for the preparation thereof

Embodiments of the present invention relate to methods, systems, and apparatus suitable for performing a survey scan of one or more analytes or labeled fragments of analytes to obtain a convoluted spectrum and to de-convolute the convoluted spectrum using, for example, a mass spectrometer and associated processing system.

Method and apparatus for de-convoluting a convoluted spectrum

Compositions and kits pertaining to analyte determination

Embodiments of the present invention relate to methods and systems suitable for de-convoluting a convoluted spectrum, such as by way of example, data obtained from a mass spectrometer.

Growing community of inventors

Boxborough, MA, United States of America

Darryl J C Pappin