Northern Arizona University

The Translational Genomics Research Institute

Darrin Lemmer

Paul S Keim

David Engelthaler

Elizabeth Driebe

Jolene Bowers

Erin Kelley

The present invention provides a method of detecting one or morespecies within a sample from a subject, the method comprising: subjecting DNA and/or RNA from the sample to a PCR amplification reaction using primer pairs targeting species-specific canonical single nucleotide polymorphisms (canSNPs); and analyzing amplification products resulting from the PCR amplification reaction to detect the one or morespecies. The present invention also provides a kit for detection of one or morespecies,clonal groups, AMR genes, and/or virulence genes, the kit comprising primer pairs targeting species-specific canSNPs,genes M1 and M2, clonal group-specific canSNPs, AMR genes, and/or virulence genes.

Methods and kits to identifystrains

An Amplicon Sequencing Analysis Pipeline (ASAP) system () characterizes a genetic sample. The ASAP system () receives assay configuration data individually associating reference sequences and genetic characteristics. The ASAP system () processes amplicon sequencing data and the reference sequences to characterize the genetic sample based on the individual associations between the reference sequences and the genetic characteristics in the assay configuration data. The ASAP () system transfers genetic data indicating the genetic characteristics for the genetic sample and indicating interpretation metrics for amplicon sequencing read depth and quality related to the genetic characteristics.

Enhanced amplicon sequencing analysis

Growing community of inventors

Flagstaff, AZ, United States of America

Darrin Lemmer