National Technology & Engineering Solutions of Sandia, LLC

Sandia Corporation

Stc.unm

University of South Florida

Unm Rainforest Innovations

Sandia National Laboratories

Saudia Corporation

Darren W Branch

Thayne L Edwards

David R Wheeler

Roy H Olsson

Kurt O Wessendorf

Richard Smith Larson

David C Brown

Susan M Brozik

Brian Hjelle

Marco Bisoffi

Kenneth Wojciechowski

Christopher Nordquist

Conrad D James

Bryan Carson

Philip Rocco Miller

DeAnna Marie Campbell

Pam R Hall

Gennifer Tanabe Smith

Harold G Craighead

Ronald P Manginell

Venkat Rama Bhethanabotla

Matt Eichenfield

Igal Brener

Paul Gilbert Clem

Thomas A Friedmann

Ihab Fathy El-Kady

Christian Lew Arrington

Charles M Reinke

Aleem Siddiqui

Sean Michael Scott

Erika Cooley Vreeland

Jennifer K Sigman

Stefan Cular

James Kenneth Douglas

Mehmet Fatih Su

Maryam Ziaei-Moayyed

Grant D Meyer

Anne Ruffing

Erika Jane Cooley

Jaime L McClain

Surendra K Ravula

Martyn Hill

Rosemary June Boltryk

The present invention relates to systems including an acoustic wave resonator and an active shunt capacitance cancelling oscillator circuit. Such systems can be used in biosensing methods, while avoiding impedance distortion and phase shift anomalies.

Acoustic wave resonator with active shunt capacitance cancellation and systems thereof

A method for detecting a change in an environment is disclosed. The method includes forming a test acoustic signal based on time-reversal of a baseline acoustic signal after the baseline acoustic signal has traversed the environment while the environment is in a baseline condition. The method also includes detecting the test acoustic signal after the test acoustic signal has traversed the environment while the environment is in an unknown condition. The method includes determining whether there has been a change in the environment by comparing the baseline acoustic signal with the detected test acoustic signal after the test acoustic signal has traversed the environment while the environment is in the unknown condition.

Time reversal acoustics method and apparatus for detecting changes in environments

The present invention relates to methods of transforming a cell by use of an acoustic transducer. In particular embodiments, the radiofrequency signal to the transducer can be tuned to provide optimal membrane disruption of the cell; and an agent, such as a nucleic acid, can then be delivered to the cell.

Methods of transfection using sonoporation

Multifunctional RF limiting amplifiers having various configurations and functions are disclosed. In a first configuration, the RF limiting amplifier includes an active load output circuit that allows one to adjust the output impedance based upon the anticipated connected load impedance. In a second configuration, the RF limiting amplifier includes a pair of emitter-followers to buffer the output of a first stage, allowing the RF limiting amplifier to drive one or more second stages. A third configuration includes a pair of RF limiting amplifiers with their outputs mixed to implement a down conversion function. The third configuration may be used to drive dual SAW resonators for detecting the presence of biological or chemical agents. The RF limiting amplifier may be implemented in either bipolar junction transistors or CMOS transistors.

Multifunctional RF limiting amplifier

The present invention relates to encapsulated microfluidic packages and methods thereof. In particular embodiments, the package includes a device, a cradle configured to support the device, and a lid having a bonding surface configured to provide a fluidic seal between itself and the device and/or cradle. Other package configurations, as well as methods for making such fluidic seals, are described herein.

Microfluidic package and method of making the same

The present invention relates to tunable microresonators, as well as methods of designing and tuning such resonators. In particular, tuning includes applying an electrical bias to the resonator, thereby shifting the resonant frequency.

Solid-state tuning behavior in acoustic resonators

Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50&#xd7;10-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

Detection of bioagents using a shear horizontal surface acoustic wave biosensor

The present invention relates to an active shunt capacitance cancelling oscillator circuit. Such systems can be used in resonator-based methods, while avoiding impedance distortion and phase shift anomalies.

Active shunt capacitance cancelling oscillator for resonators

The present application relates to a biosensor that employs an acoustic cavity to store mechanical energy. In particular examples, the biosensor includes an electrode region and one or more reflector regions to form the acoustic cavity, as well as a functionalized active area disposed in proximity to the cavity. Methods of making and using such biosensors are also described herein.

Shear horizontal surface acoustic wave (SH-SAW) resonators and arrays thereof

The present invention relates to methods for suppressing spurious modes in microresonators. In particular embodiments, such spurious modes can be reduced by providing one or more structural components that effectively reduce the acoustic velocity in the transducer domain. Additional methods and microresonators are described herein.

Methods for suppressing spurious modes in microresonators

The present invention relates to an acoustic lysis system including a disposable cartridge that can be reversibly coupled to a platform having a small, high-frequency piezoelectric transducer array. In particular, the system releases viable DNA, RNA, and proteins from human or bacterial cells, without chemicals or additional processing, to enable high-speed sample preparation for clinical point-of-care medical diagnostics and use with nano/microfluidic cartridges. Also described herein are methods of making and using the system of the invention.

Miniature acoustic wave lysis system and uses thereof

A microresonator with an input electrode and an output electrode patterned thereon is described. The input electrode includes a series of stubs that are configured to isolate acoustic waves, such that the waves are not reflected into the microresonator. Such design results in reduction of spurious modes corresponding to the microresonator.

Microresonator electrode design

An acoustically coupled frequency selective radio frequency (RF) device is provided. The device includes a piezoelectric substrate overlain by a plurality of electrodes. The device further includes a pair of RF input terminals at least one of which is electrically connected to at least one of the electrodes, and a pair of output RF terminals, at least one of which is electrically connected to at least one other of the electrodes. At least one of the electrodes is electromechanically reconfigurable between a state in which it is closer to a face of the piezoelectric substrate and at least one state in which it is farther from the face of the piezoelectric substrate.

Programmable electroacoustic filter apparatus and method for its manufacture

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Albuquerque, NM, United States of America

Darren W Branch