Bio-rad Laboratories, Inc.

Raindance Technologies, Inc.

Harvard College

Medical Research Council

United Kingdom Research and Innovation

Catalog Technologies, Inc.

The Governing Council of the Univ. of Toronto

Other

Brandeis University

National Science Foundation

University of Strasbourg

University of Toronto

Kumacheva, Eugenia

Darren Roy Link

Michael L Samuels

Qun Zhong

Jonathan William Larson

Keunho Ahn

Andrew David Griffiths

David A Weitz

Michael Weiner

Jerome Bibette

Jeffrey Charles Olson

David Marran

Benjamin J Miller

Jonathan Marc Rothberg

Yevgeny Yurkovetsky

Andrew Watson

Keith Brown

Zhengdong Cheng

Mark Weary

Andrew Wilson

Brian Hutchison

Wolfgang Hinz

Galder Cristobal-Azkarate

John Brian Hutchison

John Q Lu

Gilbert Feke

Laurent Boitard

Jeffrey Branciforte

Yves Charles

Ahmadali Tabatabai

George M Whitesides

Howard A Stone

Eugenia Kumacheva

Swapnil P Bhatia

Nathaniel Roquet

Hyunjun Park

Irina Gitlin

Willow Ruth Diluzio

Manuel Marquez-Sanchez

Shelley L Anna

Piotr Garstecki

Nathalie Bontoux

Akim Lennhoff

Patrick Odoy

Andrew S Utada

John A Luckey

Xinyu Li

Seth Fraden

Jonathan M Rothberg

Sirshendu Roopom Banerjee

Abdeslam El Harrak

Jung Uk Shim

Eric Brouzes

François Caron

Felix Kleinschmidt

Kuenho Ahn

Andrew D Griffiths

Brian Hutchinson

David Weitz

Shelly L Anna

The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

Digital analyte analysis

The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

Manipulation of microfluidic droplets

The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.

Microfluidic devices

The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

Sandwich assays in droplets

Methods and systems for encoding digital information in nucleic acid (e.g., deoxyribonucleic acid) molecules without base-by-base synthesis, by encoding bit-value information in the presence or absence of unique nucleic acid sequences within a pool, comprising specifying each bit location in a bit-stream with a unique nucleic sequence and specifying the bit value at that location by the presence or absence of the corresponding unique nucleic acid sequence in the pool. But, more generally, specifying unique bytes in a bytestream by unique subsets of nucleic acid sequences. Also disclosed are methods for generating unique nucleic acid sequences without base-by-base synthesis using combinatorial genomic strategies (e.g., assembly of multiple nucleic acid sequences or enzymatic-based editing of nucleic acid sequences).

Systems for nucleic acid-based data storage

The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.

Microfluidic devices and methods of use in the formation and control of nanoreactors

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Compositions and methods for molecular labeling

The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

Systems and methods for handling microfluidic droplets

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

Enzyme quantification

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Lexington, MA, United States of America

Darren Roy Link