Life Technologies As

Life Technologies Corporation

Invitrogen Dynal As

Darren Ellis

Kornelija Zgonc

Tine Borgen Hildisch

Richard Johan Toften

Erlend Ragnhildstveit

Gaute Brede

Lars Nokleby

Geir Fonnum

Nini Kjus

Jeremy Mundy

Jeremy (“Jez”) Mundy

Lars Noekleby

Jez Mundy

Hannah Lindstroem

Malin Karlsson

Jeremy (Jez) Mundy

Malin Karlson

A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C-Calkanediol.

Nucleic acid preparation method

The disclosure generally relates to compositions and methods for the extraction of nucleic acids from biological samples. In particular embodiments the extraction involves mixing dibromochloromethane, iodochloromethane or mixtures thereof with a biological sample that has been mixed with a phenol-based extraction solution. Centrifugation of the sample is not needed to achieve phase separation which occurs using the present methods in as little as one to two minutes with no phenol carryover.

Methods and compositions for nucleic acid isolation

A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support while maintaining it on the support. The polyol may for example be a C-Calkanediol.

Magnetic separation apparatus

The present disclosure relates to a magnetizing portion for providing a high-gradient magnetic field in a magnetic separation device. The magnetizing portion comprises at least one magnetic assembly. The at least one magnetic assembly comprises: a plurality of magnets whereby each magnet has a north pole, south pole and a magnet axis extending between the north and south poles, and the plurality of magnets are arranged one above the other in a direction at least substantially perpendicular to the axis of each magnet in such a manner that the north and south poles of adjacent magnets are arranged alternately and a space is provide between adjacent magnets; and at least one non-magnetic spacing means arranged in the space between adjacent magnets. The present disclosure also relates to magnetic separation devices comprising at least one of the said magnetizing portions and to a method of isolating magnetically labelled particles using the magnetic separation devices.

Magnetising portion for a magnetic separation device

The disclosure relates to a magnetic separation rack for isolating magnetically labeled particles from a non-magnetic medium comprising a body portion () and a foot portion (). The body portion comprises an array of sample vessel retaining portions () and plurality of magnetizing portions (). Each sample vessel retaining portion comprises at least one visible portion such that when a sample vessel is mounted in a sample vessel retaining portion at least one portion of the sample vessel is visible to a user. The magnetizing portions are arranged within the body portion () such that at least two magnetizing portions () are circumferentially spaced about each sample vessel retaining portion (). The foot portion is pivotally coupled to the body portion such that the body portion is operatively tiltable with respect to the foot portion such that each sample vessel retaining portion may retain a sample vessel mounted therein in a tilted position with respect to the vertical. The disclosure further relates to a method of isolating magnetically labeled particles from a non-magnetic medium using the said magnetic separation rack.

Magnetic separation rack

The disclosure relates to a magnetic separating device for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion () having a magnetising portion () for providing a magnetic field and a surface by means of which the body portion may stand on a supporting surface; and a sample vessel retaining portion () for retaining at least one sample vessel (), wherein, the magnetising portion () is configured to conform at least approximately to at least a substantial portion of the longitudinal profile of at least one sample vessel (); the sample vessel retaining portion () is configured to retain at least one sample vessel () such that at least a portion of the contents of the sample vessel () is visible to a user; and the sample vessel retaining portion () is configured to be mountable on the magnetising portion so that in use, the at least one sample vessel is subject to the magnetic field of the magnetising portion (). The disclosure further relates to a kit of parts and a method for isolating magnetically labelled particles from a non-magnetic medium using the magnetic separation device.

Magnetic separating device

Growing community of inventors

Lommedalen, Norway

Darren Ellis