Endotronics, Inc.

Darrell P Page

William H Harm

John R Wilson

William A Gaines, Jr

A method of culturing cells by providing a highly gassed media on a continuous basis to cells grown in a hollow fiber cartridge includes entraining the media with gas above the gas solubility level of the media. The media is then permitted to rest in a substantially quiescent state so that non-solubilized gas leaves the media. The media is then pressurized prior to delivery to the hollow fibers sufficiently so that the pressure drop and/or flow disturbance in the cell culturing device does not decrease the gas solubility level of the media below the media's gas saturation point.

Growing community of inventors

Coon Rapids, MN, United States of America

Darrell P Page