Illumina Corporation

Epicentre Technologies Corporation

Darin J Haskins

Molly Min He

Amirali Kia

Erin Bomati

Ramesh Vaidyanathan

Haiying Li Grunenwald

Scott Kuersten

Christian Gloeckner

Trina Faye Osothprarop

Joshua Burgess

Anupama Khanna

Daniel Schlingman

Leslie M Hoffman

Jerome J Jendrisak

Michael J Fiandt

Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.

Modified transposases for improved insertion sequence bias and increased DNA input tolerance

The present invention provides compositions and methods for controlling the copy number for a broad range of plasmids and uses thereof. Disclosed is a host cell for conditional control of copy number of a plasmid, which host cell comprises a poly(A) polymerase gene that is operably joined to a conditionally inducible promoter, and a method for cloning and stably maintaining a DNA sequence encoding a heterologous polypeptide in the host cell.

Compositions and methods for controlling copy number for a broad range of plasmids and uses thereof

A method for retrofitting DNA in a single-copy or high-copy vector, such as a fosmid or BAC, whereby an artificial transposon is used to introduce a conditional multi-copy origin of replication ('ori') into the DNA in said vector. Following random in vitro or in vivo transposition of the ori-containing transposon into DNA in the single-copy or low-copy vector, the resulting insertion clones are introduced into a special host strain that contains a gene which encodes a polypeptide required for replication from the multi-copy ori. However, since the gene for this polypeptide is expressed from a tightly-regulated inducible promoter, the polypeptide is not expressed in the absence of inducer. On addition of inducer to the culture medium, the host cell synthesizes the polypeptide, which in turn activates replication from the multi-copy ori, thereby increasing the amount of clone DNA synthesized by the cell.

Methods and compositions for amplifying DNA clone copy number

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Blue Mound, WI, United States of America

Darin J Haskins