Verenium Corporation

Other

Darcy Madden, Legal Representative

Dan Eric Robertson

David Paul Weiner

Jennifer Ann Chaplin

Jay Milton Short

Mark Joseph Burk

Ellen Chi

Grace DeSantis

Aileen Milan

Mark Madden

The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.

Nucleic acids encoding nitrilases

Nitrilases

Nitrilases and methods for making and using them

In accordance with the present invention there are provided methods for producing enantiomerically pure &#x3b1;-substituted carboxylic acids, such as, for example, &#x3b1;-amino acids and &#x3b1;-hydroxy acids, said method comprising combining an aldehyde or ketone with a cyanide and ammonia or an anmionium salt or an amine, in the presence of a nitrilase or a polypeptide having nitrilase activity which stereoselectively hydrolyzes the amino nitrile or cyanohydrin intermediate under conditions sufficient to produce the enantiomerically pure &#x3b1;-substituted carboxylic acid, such as, for example, &#x3b1;-amino acids and &#x3b1;-hydroxy acids.

Methods for producing &#x3b1;-substituted carboxylic acids using nitrilases and strecker reagents

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San Diego, CA, United States of America

Darcy Madden, Legal Representative