Syngenta Participations Ag

Daolong Wang

Dale Wayne Skalla

Venkata Krishna Kishore

Paul Altendorf

Vance Cary Kramer

Thomas Joseph Prest

David Jay Foster

Todd Lee Warner

Robert Lynn Miller

Sonali Dilip Gandhi

Molly Dunn

Nicolas Federico Martin

Joseph Dallas Clarke, V

Chris Zinselmeier

William Briggs

Gayle Dace

Libardo Andres Gutierrez Rojas

Meijuan Li

John Arbuckle

Kari Denise Kust

Allen Sessions

Jon Aaron Tucker Reinders

Christine Chaulk-Grace

Joseph Dallas Clarke

Ju-Kyung Yu

Jianwei Lu

Xi Chen

Joseph Richard Byrum

Michael G Muszynski

Zhigang Guo

Min Li

Mary Lynn Senior

Sarah Alissa Forrester

Librardo Andres Gutierrez-Rojas

Sonali Gandhi

Libardo Andres Gutierrez-Rojas

Joseph Byrum

Martin Nicolas

Joseph Dallas V Clarke

The presently disclosed subject matter relates to methods and compositions for identifying, selecting, and/or producing drought tolerant maize plants or germplasm. Maize plants or germplasm that have been identified, selected, and/or produced by any of the methods of the presently disclosed subject matter are also provided.

Genetic markers associated with drought tolerance in maize

Methods of identifying a single nucleotide polymorphism associated with a plant trait and methods of identifying a plant having an improved trait. The plant trait is correlated with at least one single nucleotide polymorphism in a microRNA region of a plant genome. Isolated nucleic acids, transgenic plants, and methods of producing the same are also disclosed.

MicroRNA polymorphisms conferring enhanced drought tolerance in a plant

Methods for introgressing an allele of interest of a locus associated with a yield trait intogermplasm are provided. In some embodiments, the methods include providing aplant that contains an allele of interest of a locus associated with a yield trait, wherein the locus associated with the yield trait is identifiable by PCR amplification of anucleic acid with a pair of oligonucleotides primers as disclosed herein, and introgressing the allele of interest intogermplasm that lacks the allele. Also provided are methods for identifyingplants that contain at least one allele associated with improved yield, improved maize plants, eliteplants, biomass produced from improvedplants, isolated and purified genetic markers, and compositions that include an amplification primer pair capable of amplifying anucleic acid to generate amarker amplicon.

Yield traits for maize

Provided herein are methods for evaluating associations between candidate genes and a trait of interest in a population. The methods include a combination of genome-wide association analysis and one or more of nested association mapping (NAM), expression QTL analysis (eQTL), and allele epistastic analysis (AEA). Markers are selected or prioritized if they are shown to be positively-correlated with a trait of interest using GWA and a combination of one or both of NAM and eQTL. Also provided are models for evaluating the association between a candidate marker and a trait in a nested population of organisms. These methods include single marker regression and multiple marker regression models. Markers identified using the methods of the invention can be used in marker assisted breeding and selection, as genetic markers for constructing linkage maps, for gene discovery, for identifying genes contributing to a trait of interest, and for generating transgenic organisms having a desired trait.

Method for selecting statistically validated candidate genes

Growing community of inventors

Research Triangle Park, NC, United States of America

Daolong Wang