Tianjin University of Science and Technology

Zhejiang Zhenyuan Biotech Co. Ltd.

Daoguang Tian

Xixian Xie

Heyun Wu

Yue Zhang

Weiming Fan

Jianqing Tu

Yanna Chen

Wei Jiang

A genetically engineered bacterium includes a genome of theand a mutant encoding gene hisG* of aATP phosphoribosyl transferase HisG on the genome, and the gene hisG* is strongly expressed to enhance activity of a key enzyme HisG for histidine synthesis. The gene hisG* has a nucleotide sequence as shown in SEQ ID NO: 1; a copy number of histidine operon genes hisDBCHAFI of theis further increased on the genome to enhance a terminal synthetic route of histidine; an encoding gene lysE from an arginine/lysine transportprotein of theis further integrated to the genome and strongly expressed to promote the intracellular histidine secrete to the extracellular space; and an encoding gene rocG of glutamate dehydrogenase ofis further integrated to the genome and strongly expressed to promote generation of histidine.

Genetically engineered bacterium for producing L-histidine and use thereof

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Tianjin, China

Daoguang Tian