National Institutes of Health, a Component of the US Dept. of Health & Human Services

Research Corporation Technologies, Inc.

Danuta Pieniazek

Jorge Velarde

Renu B Lal

Ronald B Luftig

Chunfu Yang

Susan B Slemenda

Norman J Pieniazek

Reagents and assays for detecting HIV-1 groups M and O and optionally HIV-1 group N and SIVcpz are provided. The reagents are nucleic acid primers for the hybridization to, amplification and subsequent detection of HIV-1 groups M, N and O and SIVcpz in a biological sample. The primers are oligonucleotides that selectively hybridize to the highly conserved regions of the env and pol regions of HIV-1. Due to the high sensitivity of the assays, small concentrations of HIV in a biological sample can be detected, allowing diagnosis at an early stage of infection. The assays are qualitative or quantitative and are useful for viral load determinations of HIV-1 groups M, N or O in a patient undergoing treatment for HIV-1 infection. Viral load determinations can be used to monitor the progress of the treatment regimen, the development of drug resistance, and to predict disease progression.

Methods and reagents for molecular detection of HIV-1 groups M, N and O

The present invention relates to DNA and proteins of human adenovirus Type 41 and their use in detection of said virus. More specifically, the present invention relates to the isolation of a 41.4 kd short fiber protein and a 60.6 kd long fiber protein of adenovirus type 41 (Ad41), as well as proteins derived from the Ad41 E3 region, thereby providing virus-derived antigens and active derivatives and parts thereof, useful in the development of diagnostic assays, DNA probes and vaccines for said virus or other related viruses belonging to the human enteric adenovirus family. In addition, the present invention is directed to recombinant DNA molecules containing the human enteric adenovirus Type 41 Tak long fiber protein gene, the Ad41 short fiber protein gene and the Ad41 E3 gene (encoding the Ad41 proteins RL-1 to RL-6) thereby providing a source of recombinant viral components useful in the development of said diagnostic assays, DNA probes and vaccines for human adenoviruses. The present invention is also directed to first antibodies specific to the above-identified Ad41 viral components and to second antibodies specific to the first antibodies. These second antibodies are also useful in the development of diagnostic assays for Ad41 and other adenoviruses.

Detection of human adenovirus

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Suwanee, GA, United States of America

Danuta Pieniazek