Hemosol Inc.

Danna Lynn Skea

David N Bell

Phyllis Robin Hedge

The method for obtaining and expanding TcR&ggr;&dgr; T cells in culture is described. The method involves: 1) culturing cells from a sample containing TcR&ggr;&dgr; T cells or precursors thereof in a first culture medium comprising a T cell mitogen and at least two cytokines and 2) culturing the cells obtained in step 1) in a second culture medium comprising at least two cytolines. Preferably, the method comprises 1) culturing the cells in a first culture medium comprising (a) a T cell mitogen, (b) interleukin-2 and (c) interleukin-4, and 2) culturing the cells obtained in step 1) in a second culture medium comprising (i) interleukin-2 and (ii) interleukin-4 to obtain TcR&ggr;&dgr; T cells. The TcR&ggr;&dgr; T cells obtained by the method can be used in a variety of experimental, therapeutic and commercial applications.

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Mississauga, Canada

Danna Lynn Skea