Noda Institute for Scientific Research

Kikkoman Corporation

Danji Fukushima

Eiichi Nakano

Narimasa Saito

Tsutomu Masuda

Masaru Matsuura

Akio Obata

Toshiaki Ariga

Norikazu Fujii

Akio Nobuhara

Ikunori Koshiyama

An antioxidant containing a proanthocyanidin which is at least one member selected from the group consisting of those dimers to decimers which have bonded thereto as a structural unit a flavan-3-ol or flavan-3,4-diol represented by the general formula: ##STR1## (wherein R.sub.1 is hydrogen or a hydroxyl group, R.sub.2, R.sub.3 and R.sub.4 are each hydrogen, a hydroxyl group or a methoxy group, and R.sub.5 is hydrogen, a galloyl group or a glycopyranosil group).

Antioxidant comprising proanthocyanidin as principal component

This invention relates to a method for grinding soybeans by grinding soaked soybeans together with a predetermined amount of water to obtain a soybean slurry which method comprises grinding the soaked soybeans in the presence of 10 to 30% by volume of air relative to the total weight of the soaked soybeans and the water, and also to a method for producing retort or aseptic packed tofu using the soybean milk obtained by the method of grinding.

Method for grinding soybeans and method for producing tofu

A method for the preparation of a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a temperate phage DNA having an endonuclease-sensitive region not in the DNA segment participating in the replication of phage DNA and the integration of phage DNA into a host chromosome but at least in the DNA segment carrying genetic information for the coat protein production and another DNA carrying intended genetic information, (2) adding DNA-ligase to the mixture of both cleft DNA's, and (3) recovering from the mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segments carrying genetic information for the coat protein production with a DNA fragment carrying the intended information.

Method for preparation of a recombinant DNA phage

A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.

Method for preparing a recombinant DNA phage

A novel bacteriophage whose DNA molecule has endonuclease-sensitivity only in the DNA region carrying genetic information for the production of phage coat proteins can be obtained by isolating an endonuclease-resistant mutant from one of the lambdoid bacteriophages and mating the resulting bacteriophage with a lambdoid phage having endonuclease-sensitivity in the DNA region carrying genetic information for the production of coat proteins.

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Omiya, Japan

Danji Fukushima