Tate & Lyle Ingredients Americas LLC

Universitá Degli Studi Di Milano-Bicocca

A E Staley Manufacturing Company

Galatea Biotech S.r.l.

Biopolo S.c.r.l.

Biopolo S.c.a.r.l.

Danilo Porro

Marina Vai

Lilia Alberghina

Bianca Maria Ranzi

Michael Sauer

Aaron Adrian Winkler

Michele Bianchi

Laura Frontali

Paola Branduardi

Luca Brambilla

Diethard Mattanovich

Minoska Valli

Nadia Maria Berterame

Stefano Bertacchi

Disclosed is the production by fermentation of poly D-lactic acid (PDLA) and poly L-lactic acid (PLLA). In particular, there is provided engineered (prokaryotic or eukaryotic) cells for the direct synthesis of PLLA polymers and engineered eukaryotic cells for the direct synthesis of PDLA polymers starting from a carbon source, including residual biomasses of the different production chains.

Cell able to produce poly l-lactic acid

Herein is disclosed a method of generating ascorbic acid from yeast. In one embodiment, the yeast is aspp. or aspp. growing in a medium comprising an ascorbic acid precursor. In a second embodiment the yeast is a recombinant yeast growing in a medium comprising an ascorbic acid precursor. Preferably the recombinant yeast is transformed with a coding region encoding an enzyme selected from L-galactose dehydrogenase (LGDH), L-galactono-1,4-lactone dehydrogenase (AGD), D-arabinose dehydrogenase (ARA), D-arabinono-1,4-lactone oxidase (ALO) or L-gulono-1,4-lactone oxidase (RGLO). The ascorbic acid precursor is preferably D-glucose, L-galactose, L-galactono-1,4-lactone, or L-gulono-1,4-lactone. In another preferred embodiment the ascorbic acid is accumulated in the medium at levels greater than background. Preferably, the yield of the conversion of the precursor to ascorbic acid is preferably at least about 35%.

Ascorbic acid production from yeast

A method of producing an organic acid by staining a yeast population with a stain capable of internal pH (pH)-dependent fluorescence, to yield a stained yeast population; determining a gate pH and a corresponding fluorescence parameter of the stained yeast population; and sorting the cells of the stained yeast population such that the cells having a pHabove the gate pH are retained and the cells having a pHbelow the gate pH are discarded, to yield a yeast population for the production of the organic acid. Also, a method of producing an organic acid by performing the above steps, followed by isolating individual cells of the yeast population, to yield individual yeast cells for the production of an organic acid; culturing an individual yeast cell, to yield a cloned yeast population for the production of an organic acid; and incubating the cloned yeast population for the production of an organic acid in a medium containing an organic acid precursor, to produce the organic acid.

Methods for selecting a yeast population for the production of an organic acid and producing an organic acid

Yeast strains transformed with at least one copy of a gene coding for lactic dehydrogenase (LDH) and further modified for the production of lactic acid with high yield and productivities, are described.

Yeast strains for the production of lactic acid

Processes for producing lactic acid using yeast transformed with a gene encoding lactate dehydrogenase

The invention refers to the production of recombinant gene products from cultures of the yeaststrains transformed with expression vectors bearing the gene coding for said proteins.

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The production of recombinant gene products from cultures of the yeaststrains transformed with expression vectors bearing the gene coding for the proteins.

Production of heterologouus proteins from Zygosaccharomyces bailii

Herein is disclosed a method of generating ascorbic acid from yeast. In one embodiment, the yeast is a Zygosaccharomyces spp. or a Kluyveromyces spp. growing in a medium comprising an ascorbic acid precursor. In a second embodiment the yeast is a recombinant yeast growing in a medium comprising an ascorbic acid precursor. Preferably the recombinant yeast is transformed with a coding region encoding an enzyme selected from L-galactose dehydrogenase (LGDH), L-galactono-1,4-lactone dehydrogenase (AGD), D-arabinose dehydrogenase (ARA), D-arabinono-1,4-lactone oxidase (ALO) or L-gulono-1,4-lactone oxidase (RGLO). The ascorbic acid precursor is preferably D-glucose, L-galactose, L-galactono-1,4-lactone, or L-gulono-1,4-lactone. In another preferred embodiment the ascorbic acid is accumulated in the medium at levels greater than background. Preferably, the yield of the conversion of the precursor to ascorbic acid is preferably at least about 35%.

Yeast strains for the production of lactic acid transformed with a gene coding for lactic acid dehydrogenase

Growing community of inventors

Milan, Italy

Danilo Porro