Ajinomoto Co., Ltd.

Danila Vadimovich Zimenkov

Tatyana Viktorovna Leonova

Vera Georgievna Doroshenko

Sergei Vladimirovich Mashko

Andrey Yurievich Gulevich

Aleksandra Yurievna Skorokhodova

Elena Vitalievna Klyachko

Alla Valentinovna Belareva

Vitaly Grigorievich Paraskevov, Legal Representative

Joanna Yosifovna Katashkina

Irina Vladimirovna Biryukova

Aleksandr Aleksandrovich Krylov

Vladimir Yurievich Ermishev

Aleksandr Dmitrievich Kivero

Natalya Igorevna Minaeva

Yury Ivanovich Koslov

The present invention provides a method for constructing recombinant translationally coupled operons, a method for producing useful metabolites using the bacterium containing the coupled operons, and a method for monitoring gene expression.

Method for constructing an operon containing translationally coupled genes

An expression control sequence which controls expression of a target gene linked downstream of the expression control sequence depending on an intracellular concentration of an amino acid, wherein in a bacterial cell which harbors a DNA construct comprising the expression control sequence, a promoter linked upstream of the expression control sequence and the target gene linked downstream of the expression control sequence, frequency of termination in the expression control sequence, of transcription starting from the promoter is lowered by increase of an intracellular concentration of an amino acid, whereby expression of the target gene increases.

Expression control sequence

The present invention provides a method for producing a non-aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genusor, which has been modified to attenuate expression of the csrA gene.

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Moscow, Russia

Danila Vadimovich Zimenkov