Becton Dickinson and Company Limited

Danielle Koffenberger

Keith Edward Thornton

Paul Madepogu

Courtney E Maus

Jason P Stevens

Keith Thornton

Methods and compositions for detection ofare disclosed herein. In some embodiments, the presence or absence ofin a sample is determined using nucleic acid-based testing methods using primers and/or probes that bind to opcA gene region of

Detection of

Nucleic acids sequences that can be used for nucleic acid amplification, for example quantitative nucleic acid amplification, are provided herein.

Internal control nucleic acids for amplification

Detection of neisseria gonorrhoeaes

A region of thepmpA gene has been identified which is useful for performing amplification assays to determine specifically whetheris present in the sample being tested. Oligonucleotides useful for detecting this gene by performing the polymerase chain reaction (PCR) are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains or serovars of, including the variant E serovar, and which does not show cross reactivity with the genomes of other microorganisms or with human DNA. In addition, the disclosed oligonucleotides can be used to in a multiplex system. This invention also contemplates a kit including oligonucleotides, and optionally other reagents, for the detection ofusing PCR.

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Assay for Chlamydia trachomatis by amplification and detection of  Chlamydia trachomatis  pmpA gene

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Stewartstown, PA, United States of America

Danielle Koffenberger