Centre National De La Recherche Scientifique

Danielle Gulino Nee Debrac

Denis S Barritault

Jose P Courty

Jacqueline Jozefonvicz Nee Dorgebray

Josette Badet Nee Genissel

Marie-Anne Dourges Nee Jacquot

The present invention relates to the application, as the stationary phase in affinity chromatography for the purification of the FGF type growth factors, of the polymers or copolymers onto which --SO.sub.3 H and --SO.sub.3 M groups are randomly bound, groups in which M denotes a physiologically acceptable metal, as well as, preferably, --SO.sub.2 R groups, in which R denotes a radical obtained by removal of a hydrogen atom from the amino group of an amino acid or an amino acid derivative. The invention relates also to the obtaining of a purified bFGF or aFGF growth factor, or a mixture of these growth factors, consisting in carrying out an affinity chromatography on a resin such as defined hereinabove, by carrying out an elution with a neutral pH buffer having an ionic strength equivalent to that of a 0.15 M to 2.5 M NaCl solution.

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Neuilly Plaisance, France

Danielle Gulino Nee Debrac