Vilmorin Et Cie

Hm.clause, Inc.

Institut National De La Recherche Agronomique (inra)

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Daniele Hosemans

Abdelhafid Bendahmane

Julie Fauve

Adnane Boualem

Christelle Troadec

Eric Lionneton

Muriel Archipiano

Agnes Vermuelen

Disclosed is a method for the production of a plant of the Cucurbitaceae family by mutating seeds to induce a mutation of 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8) and growing a plant from the mutated seed, with the plant having a androecy or androecious tendency.

Methods of obtaining androecious cucurbit plants

Plants of the Cucurbitaceae family and seeds of such plants with an androecious phenotype, whereas this sexual type is not natural in the plants, and the use of the plants and of the plant seeds of the invention. Moreover, the nucleic acid sequence responsible for androecy in the plants of the invention and on the polypeptide encoded by the nucleic acid sequence. Finally, methods of identifying the plants and the seeds.

Androecious cucurbit plants, methods of obtaining and uses of said cucurbit plants

The invention relates to a method for producing haploid H, doubled haploid HD and/or dihaploid DH plants, the HD and DH being homozygous or essentially homozygous, this method being a method such as those which come under the technique of gynogenesis induced by irradiated pollen. This method comprises a step of irradiating the reproductive material of the male parent at a dose of between 160 and 190 gamma ray and/or a step of selecting the haploid H and/or DH plants by using one or more molecular marker(s). The invention also relates to a method for producing homozygous haploid, doubled haploid and/or dihaploid plants, comprising a step of determining the appropriate irradiation dose(s) for increasing the yields of said plants according to multiple given factors such as the plant species, the genotypes of the male parent and of the female parent, the climatic and weather conditions, the time at which the fruits are harvested, the level of growth of the embryos collected with a view to the culturing thereof, the level of development of the embryos placed in culture. Moreover, the invention concerns the molecular marker(s) used in the selection step and also the haploid embryos and the dihaploid embryos obtained by means of the method of the invention, and the progeny and the seeds of the plants obtained by means of the method of the invention.

Method for producing haploid, doubled haploid and/or dihaploid plants by gynogenesis

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Angers, France

Daniele Hosemans