New England Biolabs, Inc.

Daniela Munafo

Larry A McReynolds

Larry A Mcreynolds

Methods are provided for ligating a 3' adapter and a 5&#x2032; adapter to a target polynucleotide so as to avoid adapter dimer formation. Embodiments of the methods include adding a blocking oligonucleotide after the first ligation in which a 3&#x2032; adapter is ligated to the target polynucleotide so that the blocking oligonucleotide is capable of hybridizing to excess 3&#x2032; adapter and the ligated 3&#x2032; adapter. Subsequently, a 5&#x2032; adapter is ligated to the target polynucleotide thus avoiding adapter dimer formation.

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South Hamilton, MA, United States of America

Daniela Munafo