Bio-Ker Srl

Keryos Spa

Daniela Ghisotti

Gaetano Orsini

Giancarlo Tonon

Gabriele Zuffi

Giuseppina Bestetti

Simona Cali

Simona Cali'

Novel strains of genetically modified prokaryotic micro-organisms capable of expressing polypeptides having the enzyme activity of the enzymes uridine phosphorylase (UdP) and purine nucleoside phosphorylase (PNP) are described; the strains in question can be used, both in the form of whole cells and in the form of crude or purified extracts, to catalyse transglycosylation reactions between a donor nucleoside and an acceptor base with particularly high yields. The associated plasmid vectors are also described.

Catalyzing transglycosylation using a recombinant host cell overexpressing uridine phosphorylase and purine nucleoside phosphorylase

Strains of genetically modified prokaryotic micro-organisms capable of expressing polypeptides having the enzyme activity of the enzymes uridine posphorylase (UdP) and purine nucleoside phosphorylase (PNP) are described; the strains in question can be used, both in the form of whole cells and in the form of crude or purified extracts, to catalyse transglycosylation reactions between a donor nucleoside and an acceptor base with particularly high yields. The associated plasmid vectors are also described.

Vectors, host cells, and methods for production of uridine phosphorylase and purine nucleoside phosphorylase

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Milan, Italy

Daniela Ghisotti