University of Washington

Harvard College

Cellectricon Ab

Clemson University

Lamprogen, Inc.

Other

Collectricon Ab

Micareo Inc.

Clemson Research Foundation

Daniel T Chiu

Jiangbo Yu

Changfeng Wu

Fangmao Ye

Bryant S Fujimoto

Jason S Kuo

George M Whitesides

Owe Orwar

Jason E Kreutz

Xuanjun Zhang

Yu Rong

Gloria S Yen

Noo Li Jeon

Janelle R Anderson

Wei Sun

Mattias Karlsson

Robert Michael Lorenz

Insung S Choi

Mengxia Zhao

Perry G Schiro

Peter Eriksson

Yang-Hsiang Chan

Alexander R Gansen

Yong Zhang

Johan Pihl

Wyatt C Nelson

Kent Jardemark

Jessica Olofsson

David S W Lim

Chun-Ting Kuo

Jason McNeill

Jon Sinclair

Stephan K W Dertinger

Donald Elliot Ingber

Yuhui Jin

Howard A Stone

Rebecca Jane Jackman

Insung Choi

Sui Huang

Steven J Metallo

Christopher J Wargo

Anders Lundqvist

Kai Sun

Peter B Allen

Li Wu

John Scott Edgar

Hongkai Wu

Dawn E Cohen

Gavin D M Jeffries

Dingsheng Liu

Yi-Che Wu

Oksana Cherniavskaya

J Patrick Shelby

Justin Cooper McDonald

Richard Neil Zare

Jui-Lin Chen

Cecilia Farre

Ravi Kane

Noo-Li Jeon

Wei-Feng Fang

David S Lim

Anette Stromberg

Anders Karlsson

Kimberly A Dendramis

Cooper McDonald

Philip J Reid

Hui Min Yu

Alexander Moscho

Jiasi Wang

Eleanor S Shah

Yuling Qin

Seung Ryoung Jung

Jingang Wang

Yifei Jiang

Yuanhua Cheng

Jason McNeil

Maxwell Zeigler

J Cooper McDonald

Bingyun Sun

James Patrick Shelby

Gavin Jeffries

Mingyan He

Sarah Mutch

Christopher L Kuyper

Gina S Fiorini

Thomas Schneider

Ravindra Kane

Eleanor S Johnson

Chang-Feng Wu

Peggy G Schiro

The present disclosure relates to methods, systems, and devices for performing analyses of biological nanoparticles. More specifically, the present disclosure relates to methods, systems, and devices for performing single biological nanoparticle size determination on a sample while the biological nanoparticle is in transit through a microfluidic chip. In other aspects, the present disclosure relates to methods, systems, and devices for selectively capturing biological nanoparticles on a coated planar surface, the capturing being facilitated by centrifugation.

Methods and apparatus for single biological nanoparticle analysis

The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.

Functionalized chromophoric polymer dots and bioconjugates thereof

Systems and methods for analyzing particles flowing in a channel are described. In an embodiment, the channel is configured to flow a particle through a lumen of the channel, the channel defining an interrogation window configured to allow light to pass into and out of the lumen; a light engine comprising: a first light source positioned to output first excitation light onto a first portion of the channel in the interrogation window; and a second light source positioned to output second excitation light onto a second portion of the channel in the interrogation window separate from the first portion. In an embodiment, the systems include an emission fiber bundle comprising a first emission optical fiber and a second emission optical fiber, wherein a proximal end of first emission optical fiber and second emission optical fiber are arranged in an emission fiber bundle head.

Method and apparatus for flow-based, single-particle and/or single-molecule analysis

Devices and systems for droplet generation and methods for generating droplets are described. In an embodiment, the devices and systems include a capillary configured to eject a droplet, such as in response to a voltage applied to an end of the capillary. In an embodiment, the devices and systems include a moveable stage configured to carry a multi-well plate and move the stage relative to the capillary such that the ejected droplet is selectively received by a well of the multi-well plate carried by the moveable stage.

Devices and systems for droplet generation and methods for generating droplets

The present disclosure provides nanoparticle transducers and methods of use thereof for the detection of analyte concentrations in a fluid. Nanoparticle transducers can comprise a nanoparticle, such as a Pdot, coupled to an enzyme that catalyzes a reaction with the analyte. The nanoparticle transducers further comprise chromophores that emit fluorescence that varies as a function of the concentration of one of the elements of the reaction. The nanoparticle transducer thus changes fluorescence as the analyte concentration changes, transforming analyte concentration values into fluorescence intensities. The measurement of these intensities provides a measurement of the analyte concentration. The nanoparticle transducers are biocompatible, allowing for use in vivo, for the monitoring of analyte blood concentrations such as blood glucose concentrations.

Nanoparticle transducer sensors and methods of use thereof

The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.

Polymer-silica hybrid pdots and methods of use thereof

The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.

Chromophoric polymer dots

Provided herein, among other aspects, are methods and apparatuses for ranking aliquots from a suspension containing bioparticles. In certain embodiments, the bioparticles may be cells, organelles, proteins, DNAs, debris of biological origin, microbeads coated with biological compounds, or viral particles. As such, the methods and apparatuses provided herein may be used to quantify rare cells such as circulating cancer cells, fetal cells and other rare cells present in bodily fluids for disease diagnosis, prognosis, or treatment.

Ensemble-decision aliquot ranking

Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

Methods and systems for performing digital assays using polydisperse droplets

Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.

Methods, compositions and systems for microfluidic assays

Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the detection of nucleic acids and proteins. Also provided are methods, devices, systems, and compositions for improved detection and quantification of target molecules using encoded probes. In certain aspects, the methods, devices, and systems provided herein are useful in multiplexed digital assays. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of compartmentalized volumes in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

Digital nucleic acid amplification using encoded particles

Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.

Chromophoric polymer dots with narrow-band emission

The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.

Encoded chromophoric polymer particles and methods of use thereof

Growing community of inventors

Seattle, WA, United States of America

Daniel T Chiu