University of Washington

University of Utah Research Foundation

Daniel Stetson

David Baker

Neil P King

Marc J Lajoie

Elizabeth Gray

Jacob Bale

Wesley Sundquist

Yang Hsia

Gabriel Butterfield

Joerg Votteler

Katherine DaPron

The application discloses multimeric assemblies packaging one or more active component and their use to carry out nucleic acid regulation or gene editing.

Genome editing reagents and their use

The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface ('O interface'); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex ('L domain'); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (&#x201c;M domain&#x201d;), as well as membrane-enveloped versions of the multimeric assemblies.

Polypeptide assemblies and methods for the production thereof

Growing community of inventors

Seattle, WA, United States of America

Daniel Stetson