United States of America, As Represented by the Secretary of Agriculture

Monell Chemical Senses Center

Daniel Solaiman

Richard D Ashby

Jonathan A Zerkowski

Mehmet Hakan Ozdener

Alexander Bachmanov

Thomas A Foglia

Robert A Moreau

Nereus W Gunther

Yanhong Liu

A composition comprising a bitter tastant and at least one sophorolipid and optionally a carrier; wherein the composition is edible and wherein the bitter taste of said bitter tastant is reduced. A method of reducing bitter taste attributed to a bitter tastant in an edible composition, said method comprising adding to said edible composition an effective amount of at least one sophorolipid and optionally a carrier; such that any bitter taste induced by the bitter tastant is reduced.

Compositions containing a bitter tastant and at least one sophorolipid, and methods of reducing bitter taste attributed to a bitter tastant in an edible composition

NRRL B-30761 produces monorhamnolipids with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C-C) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C-C) as the lipid moiety under static growth conditions. The cloning and sequencing of three genes and proteins involved in the biosynthesis of monorhamnose-lipid (RL) is described. Expression of two of these genes, i.e., rhlA and rhlB, together inNRRL B-30761 increases RL production by at least 10-fold. Also the generation of a recombinantNRRL B-30761 capable of synthesizing dirhamnose-lipid (RL) is described. Characterization of RL and RL produced by the recombinantNRRL B-30761 is also described.

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The isolation and characterization of a glucosyltransferase gene fromis disclosed. Use of the glucosyltransferase enzyme for the production of glucosylated sterol and hydroxyl-fatty acid substrates is also disclosed. This enzyme has broad-specificity and is useful for the production of sophorolipids both in-vivo and in-vitro.

Use of glucosyltransferase gene

A sophorolid produced by a method involving reacting a compound of formula I with a compound of formula II wherein

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Dresher, PA, United States of America

Daniel Solaiman