Oxford Nanopore Technologies Limited

Daniel Ryan Garalde

Andrew John Heron

James White

Daniel John Turner

Lakmal Nishantha Jayasinghe

James Anthony Clarke

Clive Gavin Brown

Rebecca Victoria Bowen

Graham Hall

Marion Louise Crawford

Christopher Peter Youd

David Alves

James Edward Graham

Etienne Raimondeau

Mark John Bruce

David Antoni Alves

Andy Heron

The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.

Method for nanopore RNA characterization

The invention relates to a new method of delivering an analyte to a transmembrane pore in a membrane. The method involves the use of microparticles.

Methods for delivering an analyte to transmembrane pores

The invention relates to new methods of moving helicases past spacers on polynucleotides and controlling the loading of helicases on polynucleotides. The invention also relates to new methods of characterising target polynucleotides using helicases.

Enzyme stalling method

The invention relates to a new method of characterising two or more target polynucleotides using a pore. The method involves sequentially attaching to a first polynucleotide one or more subsequent polynucleotides to form a concatenated polynucleotide.

Method of nanopore sequencing of concatenated nucleic acids

Method for nanopore RNA characterisation

The invention relates to a new method of characterizing a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.

The invention relates to new methods of moving helicases past spacers on polynucleotides and controlling the loading of helicases on polynucleotides. The invention also relates to new methods of characterizing target polynucleotides using helicases.

Growing community of inventors

Oxford, United Kingdom

Daniel Ryan Garalde