Smithkline Beecham Corporation

Other

Replidyne, Inc.

Promega Corporation

Smithkline Beechman Corporation

Daniel Robert Gentry

Rebecca Claire Greenwood

Elizabeth Jane Lawlor

David John Payne

Stewart Campbell Pearson

John Timothy Lonsdale

John Edward Hodgson

David John Holmes

Karen A Ingraham

Min Wang

Martin Karl Russel Burnham

Deborah Dee Jaworski

Martin Karl Burnham

Stephanie Van Horn

Christine Marie Debouck

Jeffrey L Mooney

Richard W Carter

Elizabeth J Lawlor

Yi Yi Zhong

Nigel Grinter

Glenn Scott Van Aller

Martin Rosenburg

Philip David Hayes

John Timothy Londsdale

Lisa Kathleen Schilling

Methods for specific RNA capture, detection and quantification are presented utilizing a protein that selectively binds RNA:DNA hybrids, preferably an RNase H that is modified to reduce degradation of the nucleic acid molecules and enhance specific detection of mixed RNA:DNA nucleic acid hybrids. Labeling of the RNA and/or amplification is not required to perform these methods. Modified RNase H enzymes useful in such methods are disclosed.

Methods of capturing, detecting and quantifying RNA:DNA hybrids and a modified RNase H useful therein

The invention provides metRS2 polypeptides and polynucleotides encoding metRS2 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are preferred methods for utilizing metRS2 polypeptides and polynucleotides as diagnostic reagents and in diagnostic assays to screen for microbial infections in organisms and infections in materials.

metRS2

The invention provides metRS2 polypeptides and polynucleotides encoding metRS2 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are preferred methods for utilizing metRS2 polypeptides and polynucleotides as diagnostic reagents and in diagnostic assays to screen for microbial infections in organisms and infestations in materials.

The invention relates to metRS polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques. Also disclosed are methods for utilizing metRS polypeptides and polynucleotides in diagnostic assays.

tRNA synthetases, metRS

The invention provides FabH polypeptides and polynucleotides encoding FabH polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing FabH polypeptides to screen for antibacterial compounds.

FabH

The invention provides trpS polypeptides and DNA (RNA) encoding trpS polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing trpS polypeptides to screen for antibacterial compounds.

TrpS

spo-rel polypeptides and DNA (RNA) encoding such spo-rel and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such spo-rel for the treatment of infection, particularly bacterial infections. Antagonists against such spo-rel and their use as a therapeutic to treat infections, particularly bacterial infections are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of spo-rel nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides encoding the relA/spoT gene family and for detecting the polypeptide in a host.

Spo-rel

Tryptophenyl tRNA synthetase from Streptococcus pneumoniae

The invention provides spo/rel polypeptides and DNA (RNA) encoding spo/rel polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing spo/rel polypeptides to screen for antibacterial compounds.

Spo-rel from streptococcus pneumoniae

FabD polypeptides and DNA (RNA) encoding such FabD and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such FabD for the treatment of infection, particularly bacterial infections. Antagonists against such FabD and their use as a therapeutic to treat infections, particularly bacterial infections are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of FabD nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides encoding Fab (Fatty acid biosynthesis) and for detecting the polypeptide in a host.

FabD

The invention provides greA polypeptides and DNA (RNA) encoding greA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing greA polypeptides to screen for antibacterial compounds.

GreA from Staphylococcus aureus

The invention provides spo/rel polypeptides and DNA (RNA) encoding spo/rel polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing spo/rel polypeptides to screen for antibacterial compounds.

DNA encoding SPO/REL polypeptides of streptococcus

Tryptophanyl tRNA synthetase, trpS, of Streptococcus pneumoniae

The invention provides hisS polypeptides and DNA (RNA) encoding hisS polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing hisS polypeptides to screen for antibacterial compounds.

Histidyl tRNA synthetase from Streptococcus pneumoniae

greA polypeptides and DNA (RNA) encoding such greA and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such greA for the treatment of infection, particularly bacterial infections. Antagonists against such greA and their use as a therapeutic to treat infections, particularly bacterial infections are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of greA nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides encoding greA/greB gene family and for detecting the polypeptide in a host.

Growing community of inventors

Pottstown, PA, United States of America

Daniel Robert Gentry