Institute for Environmental Health, Inc.

Sample6 Technologies, Inc.

Phage Diagnostics, Inc.

Daniel Robert Brownell

Michael Koeris

Jayson Linn Bowers

Robert Patrick Shivers

Jason Wyatt Holder

Edyta Krzymanska-Olejnik

Michael Cappillino

Timothy Kuan Ta Lu

Gregory McKenzie

Brett Chevalier

Julie Thomason

Michael Sandor Koeris

Gregory John McKenzie

Timothy Kuan Lu

Brett Smith Chevalier

Gregory John Mckenzie

Methods of detecting target bacteria are provided. In some embodiments, the methods comprise exposing a sample to a phage capable of infecting target bacteria, said phage comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments, the target bacteria comprise. In some embodiments, the target bacteria are all. Recombinantphage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.

Recombinant phage for listeria detection

Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe include is

Codon optimized recombinant phage and methods of using same

Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise. In some embodiments the target bacteria are all. Recombinantphage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.

Phage-based bacterial detection assay

Methods of making recombinant phage, compositions and articles of manufacture of same for bacterial detection

This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.

Recombinant phage and methods

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Arlington, MA, United States of America

Daniel Robert Brownell