Li-cor, Inc.

Pacific Biosciences of California, Inc.

Daniel R Draney

Xinshe Xu

William M Volcheck

Narasimhachari Narayanan

Ananda G Lugade

Jiyan Chen

Joy Kovar

Lael Cheung

John G K Williams

Vassil Elitzin

Katie Schaepe

D Michael Olive

Xinzhan Peng

Kousik Kundu

Mark Cradduck

Garrick Murray Little

Charles Prescott

Garrick M Little

A method for organ imaging, comprising: administering to a subject a diagnostic effective amount of 2-((E)-2-((E)-3-(2-((E)-3,3-dimethyl-5-sulfonato-1-(4-sulfonatobutyl)indolin-2-ylidene)ethylidene)-2-phenoxycyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate or 2-((E)-2-((E)-3-(2-((E)-3,3-dimethyl-5-sulfonato-1-(4-sulfonatobutyl)indolin-2-ylidene)ethylidene)-2-(4-sulfonatophenoxy)cyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(4-sulfonatobutyl)-3H-indol-1-ium-5-sulfonate. In one embodiment, the organ includes one or more of kidney, bladder, liver, gall bladder, spleen, intestine, heart, lungs and muscle.

IR dyes for fluorescence imaging

Compounds are disclosed that are useful for noninvasive imaging in the near-infrared spectral range. Bioconjugate cyanine compounds of Formula II are presented: wherein Q is a portion of a polymethine bridge selected from the group consisting of: 

Bioconjugates of cyanine dyes

Compounds and methods are disclosed that are useful for noninvasive imaging in the near-infrared spectral range. The cyanine compounds of Formula I are presented:   Also included are bioconjugates of the compounds of Formula I, methods of labeling biomolecules with the compounds, and methods of imaging.

Fluorescent imaging with substituted cyanine dyes

Compounds and methods are disclosed that are useful for noninvasive imaging in the near-infrared spectral range. The cyanine compounds of Formula I are presented: wherein Q is a portion of a polymethine bridge selected from the group consisting of: Also included are bioconjugates of the compounds of Formula I, methods of labeling biomolecules with the compounds, and methods of imaging.

Cyanine dyes and their conjugates

Compounds and methods are disclosed that are useful for noninvasive imaging in the near-infrared (NIR) spectral range. The NIR is highly sensitive for tumor detection and tracking. The application discloses targeting a tumor-enriched cell surface receptor with a ligand-conjugated fluorescent probe, which specifically allows detection of the tumor relative to the negligible animal autofluorescence.

Optical fluorescent imaging

The present invention provides for cyanine dyes as near IR quenchers. The cyanine dyes have absorption wavelengths in the near-infrared region of about 650-900 nm and are essentially non-fluorescent. The dyes of the invention have at least one linking group. The present invention also provides substantially non-fluorescent, NIR probes. Biological assays based on these novel, substantially non-fluorescent, NIR probes are also provided.

Cyanine dyes and methods of use

The present invention provides compositions and methods for detecting incorporation of a labeled nucleotide triphosphate onto the growing end of a primer nucleic acid molecule. The method is used, for example, to genotype and sequence a nucleic acid. In a preferred embodiment, the method described herein detects individual NTP molecules.

DNA intercalating agents

A compound of the formula (I) wherein the variables are defined herein. Methods of dye-labeling biomolecules with the compound of formula (I) and dye-labeled biomolecules are also provided.

Cyanine dyes

DNA intercalating agents and methods of use

Fluorescent dyes are disclosed which are useful as reporter groups for labeling biomolecules. The silicon phthalocyanine dyes disclosed are preferably water soluble, isomericly pure, possess high quantum yield, and are useful in bioassays.

Phthalocyanine dyes

A compound of the formula (I)wherein Z is O, S, or NRwherein Ris H or alkyl; R&#x2013;Rare each independently H, alkyl, halo, carboxyl, amino, or SOCat, wherein Catis a cation and at least one of R&#x2013;Ris SOCat; Rand Rare each H, alkyl, or optionally, together with the (a) group to which they are bonded, form a ring; m and n are each independently integers from 0 to 5; X and Y are each independently O, S, Se or CRR, wherein Rand Rare each independently alkyl, or optionally form a ring together with the carbon atom to which they are bonded; Rand Rare each independently alkyl, (CH)Ror (CH)R; wherein at least one of Rand R(CH)Rand wherein r is an integer from 1 to 50, and Ris a functional group that does not directly react with a carboxyl, hydroxyl, amino, or a thiol group, and Ris a functional group that can react with a carboxyl, hydroxyl, amino, or thiol group; and R&#x2013;Rand R&#x2013;Rare each independently H, alkyl, halo, amino, sulfonato, RCOOH, ROR, RSR, or RCOORwherein Ris a bond or alkylene and Ris alkyl, or optionally Rand Rtogether with the atoms to which they are bonded form an aromatic ring, or optionally Rand Rtogether with the atoms to which they are bonded form an aromatic ring. Methods of dye-labeling biomolecules with the compound of formula (I) and dye-labeled biomolecules are also provided.

Growing community of inventors

Lincoln, NE, United States of America

Daniel R Draney