E.i. Dupont De Nemours and Company

Qualicon Diagnostics LLC

Daniel R Demarco

Richard C Ebersole

Rick Weizhang Ye

Mark Anton Jensen

Laura Anne Bedzyk

Stephen Varkey

Raymond E Jackson

Seth L Blumerman

This invention relates to a rapid method for detection and characterization ofbacteria serotype O157:H7 based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detectO157:H7 in a food or water sample, such as a beef enrichment. The present invention further relates to replication compositions and kits for carrying out the method of the present invention.

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This invention relates to a rapid method for detection and characterization of STEC bacteria based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. This method is preferably employed to detect STEC bacteria in a food or water sample, such as a beef enrichment. The present invention further relates to isolated polynucleotides, replication compositions, kits, and reagent tablets for carrying out the method of the present invention.

Sequences and their use for detection and characterization of STEC bacteria

This invention relates to a rapid method for detection ofin a sample based on the presence of nucleic acid sequences, in particular, to a PCR-based method for detection, and to oligonucleotide molecules and reagents and kits useful therefore. In certain embodiments, the method is employed to detectin a food or water sample. The present invention further relates to isolated polynucleotides, replication compositions, and kits for carrying out the method of the present invention.

Sequences and their use for detection of salmonella

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Wilmington, DE, United States of America

Daniel R Demarco