10x Genomics, Inc.

Daniel P Riordan

Zachary Bent

Paul Ryvkin

Jessica Michele Terry

Elliott Meer

Niranjan Srinivas

Michael Schnall-Levin

Preyas Shah

Alex Gagnon

The present disclosure provides methods of generating supports (e.g., beads) comprising barcode molecules coupled thereto. A barcode molecule coupled to a support may comprise a barcode sequence and a functional sequence. A barcode molecule may be generated using two or more ligation reactions in a combinatorial fashion. A support comprising two or more different barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.

Functionalized gel beads

The present disclosure provides methods and systems for nucleic acid preparation and analysis. Nucleic acid molecules may be derived from one or more cells. Preparation of nucleic acid molecules may comprise generation of one or more mutations, such as strand-specific mutations. Nucleic acid molecules may be prepared for and analyzed by sequencing. Sequences may be identified with nucleic acid orientation information.

Systems and methods for nucleic acid preparation and analysis

Growing community of inventors

Hillsborough, NC, United States of America

Daniel P Riordan