Biomerieux, Inc.

Biomerieux Sa

Institut Textile De France

Institut Textile De France and Bio Merieux

Daniel Monget

Arthur James

Sylvain Orenga

Roger Chatelin

Rosaleen Joy Anderson

Paul William Groundwater

Patrick Gayrine

Andrey Victorovich Zaytsev

Catherine Fitzer-Couturier

Thierry Pollet

John Perry

Denis Robichon

Lyle Armstrong

Celine Roger-Dalbert

Michel Peyret

Mireille Desmonceaux

Francois Villeval

The invention relates to a method for detecting and/or identifying() in a biological sample, that comprises inoculating the biological sample liable to containon a detection medium that comprises tryptophan and a substrate for an enzyme A, expressed by the majority of, in order to obtain bacterial colonies; detecting the colonies expressing the activity of the enzyme A and identifying them as being; and detecting the colonies that do not express the activity of the enzyme A, carrying out an indole test, and identifying the colonies having a positive indole test as being

Medium for detecting and/or identifying bacteria

Enzymatic phenoxazinone substrates of formula (I): in reaction media containing the same are used for detecting, identifying and/or quantifying microorganisms expressing at least one peptidase activity.

Enzymatic substrates derived from phenoxazinone and their use as developers in detection of microorganisms with peptidase activity

The present invention relates to a reaction medium for-group(and) andbacteria, comprising The invention also relates to the use of this medium for isolating and identifying-group(and) and Finally, the invention relates to a method for identifyingandbacteria, according to which beta-galactosidase activity is detected for identifyingand the acidification of a sugar is detected for revealing

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Novel enzymatic substrates of the general formula below: 

Enzymatic substrates derived from phenoxazinone and their use as developer in detection of microorganisms with peptidase activity

An enzyme substrate which includes a target portion and a marker portion, hydrolysis of the substrate leading to separation of the target portion from the marker portion, and the target portion being specific for the enzyme activity being assayed. A formulation containing at least one such substrate, and a method for detecting the bacterial speciesis also disclosed. The target portion of the substrate is specific for &#x3b2;-alanine aminopeptidase activity and the marker portion is a compound which reveals whether a hydrolysis reaction has taken place or not. The invention is particularly applicable in the field of diagnosis.

Enzymatic substrates for detecting Pseudomonas aeruginas

A nitrocoumarin compound or one of its derivatives which are fluorescent in the reduced state, and the use of these compounds for detecting the presence or absence of at least one microorganism. The invention concerns the use of these compounds, methods for isolating and detecting microorganisms or a group of microorganisms in a sample likely to contain them, and various applications thereof. Also disclosed is a method for detecting nitroaryl reductase activity in a bacteria culture medium.

Fluorescent detection method for microorganisms based on nitrocoumarins

The invention concerns a chromogenous substrate for detecting the presence of at least one enzyme enzymatic activity. The invention also concerns a method for using such a substrate. The invention further concerns a method for identification based on such substrates and a device for implementing said identification method. The invention is characterized in that the substrate consists of at lease two molecules, a first molecule consisting of a non-chromogenous marker part associated with at least a specific target part for the enzyme and a second molecule consisting of a non-chromogenous part, and the non-chromogenous marker part, once it is released, reacts with the second molecule to form a chromogenous molecule. The invention is particularly applicable in bacteriology.

Chromogenic substrates for detecting bacterial hydrolases

Method for adsorbing antimicrobial agents contained in a biological

Device for the physicochemical separation of constituents of a fluid

The invention relates to a method of bacteriological analysis for the selective detection of Salmonella and a medium for its implementation. The method is based on the specific capacity of Salmonella to ferment glucuronic acid or one of its salts, and not to produce .beta.-galactosidase. The medium comprises a nutrient which is metabolized by the bacteria, a chromogenic or fluorigenic compound capable of being hydrolyzed by the enzyme .beta.-galactosidase, glucuronic acid or one of its salts and a pH indicator.

Method of bacteriological analysis, and medium for the detection of

A method for the detection of microorganisms brings a sample suspected of containing a microorganism into contact with an aqueous phase reaction medium containing a carbon source and a resorufin marker. The reaction medium is incubated with the sample suspected of containing a microorganism and the fluorescence of the marker during or after incubating is observed. An observed decrease in emitted light indicates the presence of a microorganism.

Method and reagents for the detection of microorganisms

The invention relates to a process of bacteriological analysis for differentiating the pathogenic monocytogenes species in a sample which may contain bacteria of the listeria genus, a medium for its implementation and a device for identifying Listeria monocytogenes. The process consists in bringing the sample to be analyzed into contact with an identification medium comprising a chromogenic or fluorigenic substrate capable of being hydrolyzed by glycine aminopeptidase.

Process and medium for identification of bacteria of the listeria genus

A microbiological analysis cup having a flat bottom, a wall with a circular section connected to this flat bottom and an open end. Moving from the flat bottom wall of the cup, there are at least three superposed tapered zones whose respective base diameters increase from the flat bottom to the open end. The cup makes it possible to work with very small volumes of bacterial suspension for short incubation periods, which makes it very economical to use.

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Saint-Sorlin-en-Bugey, France

Daniel Monget