Cornell University

Daniel M Knowles

Ethel Cesarman

Leandros Arvanitakis

Enrique Mesri

The present invention is directed to isolated nucleic acid molecules encoding proteins of Kaposi's sarcoma associated herpesvirus, including an antigenic receptor protein, a G protein coupled receptor, and a cyclin protein. Expression vectors and host cells comprising the nucleic acid molecules are also provided, as well as methods for increasing or decreasing the expression of the KSHV proteins in host cells. DNA oligomers and antibodies specific for the KSHV proteins are provided, each of which can be used to detect the KSHV proteins in a sample. Isolated KSHV proteins are also provided.

DNA encoding proteins of Kaposi's sarcoma associated herpesvirus

The invention provides a cell line comprising Kaposi's sarcoma-associated herpesvirus (KSHV). Preferably, the cell line is a body cavity-based lymphoma cell line, and more preferably, the cell line does not harbor Epstein-Barr virus. Two cell lines designated BC-2 and BC-3 are thus provided. These cell lines can be used in a method of propagating KSHV. The method comprises culturing the cell line, wherein the KSHV within the cell line thereby propagates. The BC-3 cell line, in particular, can be used in a method of propagating KSHV in the absence of Epstein-Barr virus. The method comprises culturing the cell line designated BC-3, wherein the KSHV within the cell line thereby propagates. Further provided is a purified viral suspension of KSHV, as well as a composition comprising purified KSHV and a suitable carrier.

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Forest Hills, NY, United States of America

Daniel M Knowles