Emulate, Inc.

Harvard College

Brown University

S.d. Sight Diagnostics Ltd.

Cedars-sinai Medical Center

Other

Vanderbilt University

University of Ottawa

Digital Lightcircuits, Inc.

Janssen Biotech, Inc.

Daniel Levner

Christopher David Hinojosa

Norman Wen

Geraldine A Hamilton

Donald Elliot Ingber

Josiah Daniel Sliz

Antonio Varone

S Jordan Kerns

Jacob Fraser

Joshua Gomes

Catherine Karalis

Kyung Jin Jang

Guy Robert Thompson, Ii

Jose Fernandez-Alcon

Michael Super

Justin Nguyen

George M Church

Riccardo Barrile

Martin F Fay

Clive N Svendsen

Dhruv Sareen

David Conegliano

Jehyuk Lee

Jefferson Puerta

Carol Lucchesi

Guy Thompson

Jingming Xu

Carolina Lucchesi

Dongeun Huh

Joseph Joel Pollak

Noam Yorav-Raphael

Yonatan Bilu

Richard Novak

Hyoungshin Park

Andries Van Der Meer

Monicah Otieno

Kevin Kit Parker

Josue A Goss

Lewis Rowe

Matthew Daniel Solomon

Remi Villenave

Gad Vatine

Anthony Bahinski

Robert Cunningham

Petrus Wilhelmus Martinus Van Ruijven

Christian Alexander Potzner

Patrick Sean Tuohy

Robert N Barrett

Je-Hyuk Lee

Ido Bachelet

Magdalena Kasendra

Jacob Freake

Doug Sabin

Samuel Sances

Stephen R Targan

Michael Workman

Uthra Rajamani

Lina Williamson

Lori McPartlin

Youngjae Choe

Hubert Geisler

Jeffrey N Weiss

Yuval Greenfield

Lian Leng

Jonathan C Bloch

Brian Zuckerman

Tanvi Shroff

Grace Ahn

Karel Domansky

Victor Antontsev

Jonathan Rubins

Guy Thompson, Iii

John P Wikswo

Ronald S Reiserer

Mark J Cartwright

Alexander L Watters

Arnon Houri Yafin

Dmitry A Markov

Martin M Rottman

Philip C Samson

David Edward Cliffel

Lisa J McCawley

Hanan Anis

Frank Emmanuel Block

Georg Bauer

Alfred Paris

John A Mclean

Jennifer Robin McKenzie

Kambez Hajipouran Benam

Abhishek Jain

Francois Boudreault

Andries D Van Der Meer

Samuel Sanees

Arnon Yafin

Daniel Patterson

John Samuel Workman

Josh Gomes

Miles Ingram

Tiama Hamkins-Indik

David James Coon

Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.

Removing bubbles in a microfluidic device

The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.

Microfluidic model of the blood brain barrier

Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.

Systems and methods for growth of intestinal cells in microfluidic devices

A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

Open-top microfluidic devices and methods for simulating a function of a tissue

The present invention is related to the field of microfluidics and compound distribution within microfluidic devices and their associated systems. In one embodiment, present invention aims to solve the problem of molecule and compound absorbency into the materials making up laboratory equipment, microfluidic devices and their related infrastructure, without unduly restricting gas transport within microfluidic devices.

Compound distribution in microfluidic devices

Development of spinal cord on a microfluidic chip

The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

Integrated human organ-on-chip microphysiological systems

An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

Organomimetic devices and methods of use and manufacturing thereof

Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

Systems and methods for cell culture device interconnection and fluidic device interconnection

Apparatus and methods are described including successively acquiring a plurality of microscopic images of a portion of a blood sample, and tracking motion of pixels within the successively acquired microscopic images. Trypomastigote parasite candidates within the blood sample are identified, by identifying pixel motion that is typical of trypomastigote parasites. It is determined that the blood sample is infected with trypomastigote parasites, at least partially in response thereto. An output is generated indicating that that the blood sample is infected with trypomastigote parasites. Other applications are also described.

Apparatus and method for analyzing a bodily sample

Methods of analyzing nucleic acids of a cell are provided.

Compositions and methods for analyte detection

The invention generally relates to a microfluidic platforms or 'chips' for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.

Devices, systems and methods for inhibiting invasion and metasases of cancer

Growing community of inventors

Brookline, MA, United States of America

Daniel Levner