Gen-probe, Inc.

Other

Qualigen, Inc.

Daniel L Kacian

Nick Mark Carter

Nanibhushan Dattagupta

Mordi I Iheme

Mark R Kennedy

Bruce W Anderson

Sherrol Hoffa McDonough

Diane L McAllister

Timothy J Fultz

Thomas B Ryder

Kenneth A Browne

Gus G Tseo

Shirley J Johnson

Nancy Lau Shen

James Garfield Putnam

Norman C Nelson

James P Light, Ii

Paul Douglas Stull

Marc Spingola

David Bruce Lackey

Kathleen A Dickey

William M Davis

Philip W Hammond

James Russell

C Nagaraja Sridhar

Michael Garth Riggs

Lizhong Dai

Richard C Harvey

Kiyotada Nunomura

Kathleen A Clark

Elizabeth R Billyard

Frank R Gonzales

Maria R Castillo

Karen W Shannon

Mark E Filipowsky

Margarita B Kaminsky

Jason A Taylor

Jason Taylor

David E Kohne

Richard S Lee

Matthew J Scott

Scott S Breidenthal

Robert F Scalese

Jorge Velarde

Sara H Fan

Keiichi Kamisango

Nick M Carter

Janice J Clymer

Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection.

Methods for quantitative amplification and detection over a wide dynamic range

The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.

Solutions, methods and kits for deactivating nucleic acids

Reaction mixtures for quantitative amplification and detection over a wide dynamic range

A cap having a core structure dimensioned to receive a pipette therethrough. The cap includes two axially aligned frangible seals that are affixed to the core structure in a spaced-apart relationship. The frangible seals are constructed so that air passageways are formed between the frangible seals and a pipette tip when the pipette tip penetrates the frangible seals. The cap optionally includes a filter interposed between the first and second frangible seals.

Penetrable cap

Method of making a formulation for deactivating nucleic acids

A cap having a core structure dimensioned to receive a pipette therethrough. The cap includes two axially aligned frangible seals that are affixed to the core structure in a spaced-apart relationship. The frangible seals include a foil layer and are constructed so that air passageways are formed between the frangible seals and a pipette tip when the pipette tip penetrates the frangible seals. The cap optionally includes a filter interposed between the first and second frangible seals.

Methods and compositions for quantitative amplification and detection over a wide dynamic range

A cap having a plurality of rib structures situated on an inner wall of the cap to facilitate the formation of air passageways as the cap is penetrated by a pipette tip. The air passageways aid in venting air from a fluid-holding vessel closed with the cap.

Penetrable cap having rib structures

A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.

Receptacles for storing substances in different physical states

A method for obtaining the contents of a fluid-holding vessel having a cap secured to an open end thereof. The method includes penetrating a surface of the cap with a fluid transfer device and forming one or more air passageways between the penetrated surface of the cap and the fluid transfer device to allow air to be vented from the vessel. At least a portion of the contents of the vessel is then drawn into the fluid transfer device before the fluid transfer device is removed from the vessel.

Method for accessing the contents of a closed collection device

A cap including a penetrable material adapted to be pierced by a fluid transfer device and an absorbent material positioned above the penetrable material. The absorbent material permits passage of the fluid transfer device and absorbs fluid material present on an outer surface of the fluid transfer device as it is being removed from the cap. The cap is useful for accessing the contents of an associated fluid-holding vessel multiple times.

Collection device including a penetrable cap having an absorbent pile fabric

Method for obtaining a fluid from a collection device assembled to isolate a specimen retrieval device from the pathway of a fluid transfer device used to penetrate a cap of the collection device and to draw and remove the fluid from the collection device for analysis.

Method for accessing the contents of a closed vessel containing a specimen retrieval device

Growing community of inventors

San Diego, CA, United States of America

Daniel L Kacian