Electronic Biosciences, Inc.

General Electric Company

Electronics Biosciences, Inc.

Daniel K Lathrop

Andrew Dennis Hibbs

Christopher Williams Crowley

Geoffrey A Barrall

Eric N Ervin

A method of processing sequencing data obtained with a polymer sequencing system identifies the most likely monomer sequence of a polymer, regardless of stochastic variations in recorded signals. Polymer sequencing data is recorded and two or more distinct series of pore blocking signals for a section of the polymer are recorded. A value is assigned to each series of pore blocking signals to obtain multiple trial sequences. The probability that each of the trial sequences could have resulting in all of trial sequences is calculated to determine a monomer sequence with the highest probability of resulting in all of the trial sequences, termed the first iteration sequence. The first iteration sequence is systematically altered to maximize the combined probability of the first iteration sequence leading to all the trial sequences in order to obtain a most likely sequence of monomers of the polymer.

Method for deducing a polymer sequence from a nominal base-by-base measurement

An electrolytic system includes an analyte chamber having an access port for introducing a sample containing a molecules of interest, such as DNA. Electrodes create an electric field along a length of the analyte chamber to drive molecules toward an interaction region containing a nanopore, thereby increasing the arrival rate of molecules at the nanopore. Additional electrodes may be utilized to create an electric field through the nanopore to drive a molecule into the nanopore. A current sensor may be utilized to count, discriminate or characterize the molecules as they interact with the nanopore. Advantageously, system can be utilized for unamplified DNA sequencing.

System and method for increasing polymer/nanopore interactions

An inspection system includes an electromagnetic shield having electrically conductive sidewalls spaced from one another. The shield also includes a conductive third wall which spans the distance between the sidewalls, and is electrically coupled to the sidewalls. The inspection system also has an inductive sensor positioned within the electromagnetic shield. The inductive sensor has two current branches, which exhibit anti-symmetric current flow. Typically, the two current branches are positioned on opposing sides of the medial plane of the electromagnetic shield.

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San Diego, CA, United States of America

Daniel K Lathrop