Wisconsin Alumni Research Foundation

Daniel K Forrest

David Charles Schwartz

Rodney Runnheim

Michael A Newton

Konstantinos Dimitrios Potamousis

Christopher P Churas

Shiguo Zhou

Steven J Goldstein

Konstantinos D Potamousis

Rodney A Runnheim

There is provided a high throughput automated single molecule image collection and processing system that requires minimal initial user input. The unique features embodied in the present disclosure allow automated collection and initial processing of optical images of single molecules and their assemblies. Correct focus may be automatically maintained while images are collected. Uneven illumination in fluorescence microscopy is accounted for, and an overall robust imaging operation is provided yielding individual images prepared for further processing in external systems. Embodiments described herein are useful in studies of any macromolecules such as DNA, RNA, peptides and proteins. The automated image collection and processing system and method of same may be implemented and deployed over a computer network, and may be ergonomically optimized to facilitate user interaction.

Automated imaging system for single molecules

Methods are provided for ascertaining the sequence of a genomic DNA sample by nicking the DNA sample with a restriction nicking enzyme, followed by nick translation with labeled nucleotides, such that the labeled nucleotides can be quantified and compared to a known, reference genome.

Methods of whole genome analysis

Growing community of inventors

Fitchburg, WI, United States of America

Daniel K Forrest