Energy Biosystems Corporation

Haarmann & Reimer Gmbh

Glycos Biotechnologies, Inc.

Molecular Logix, Inc.

University of Notre Dame Du Lac

Institute of Gas Technology

Glycosbio Inc.

Daniel J Monticello

Charles H Squires

Kevin A Gray

Paul Campbell

Sebastian Bredow

Guo-Wei Xu

Kenneth W Mitchell

Steven W Johnson

Philip T Pienkos

Stephanie Doneske

Huaijin Zhou

John J Kilbane, Ii

John D Childs

William M Haney, Iii

Lei Xi

Charles F Kulpa

Terry F Farver

Charles Hazan

Jean-Michel Colin

Aldis Darzins

A Katrina Loomis

Mark W Collison

Werner J Bussman

Christine A McDonald

Paula J Herald

Jeffrey S Bishop

Phillip R Gibbs

James C Chen

Werner J Bussmann

Christine A Mcdonald

Compositions and methods are provided for incorporating processed oils with high monoacylglyceride (MAG) content into products and food products. Methods are specifically provided for generating high MAG content processed oils.

Method of making monoacylglyceride oils and food products containing monoacylglyceride oils

The present invention provides a novel biosynthetic pathway for the production of isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol. Further embodiments provide non-naturally occurring microorganism that have been modified to produce isoprene from 3-methyl-2-buten-1-ol or 2-methyl-3-buten-2-ol and methods of producing isoprene using said microorganism.

Microorganisms and processes for the production of isoprene

The present invention features human epidermal receptor (HER) antagonists. These antagonists are polypeptide variants of ligands of HER. The HER ligand polypeptide variants of the invention possess Pan-HER antagonistic properties and can inhibit at least one HER-mediated biological activity of one or more HER subtypes, such as inhibition of the receptor's kinase activation activity and subsequently, cell proliferation. Such polypeptide variants, and nucleic acids encoding these polypeptide variants can be used therapeutically in situations in which inhibition of HER activity is indicated.

Pan-HER antagonists and methods of use

The present invention features epidermal growth factor receptor (EGFR) antagonists. These EGFR antagonists are polypeptide variants of ligands of EGFR. The EGFR ligand polypeptide variants of the invention possess EGFR antagonistic properties and can inhibit at least one EGFR-mediated biological activity such as inhibition of the receptor's kinase activation activity and subsequently, cell proliferation. Such polypeptide variants, and nucleic acids encoding these polypeptide variants can be used therapeutically in situations in which inhibition of EGFR activity is indicated.

Epidermal growth factor receptor antagonists and methods of use

The present invention relates to a method for the desulfurization of a fossil fuel containing one or more organosulfur compounds. In one embodiment, the method comprises the steps of (1) contacting the fossil fuel with a biocatalyst capable of converting the organosulfur compound to an oxyorganosulfur compound which is separable from the fossil fuel; and (2) separating the oxyorganosulfur compound from the fossil fuel. The oxyorganosulfur compound can then be isolated, discarded or further processed, for example, via desulfurization by a biocatalyzed process or an abiotic process, such as hydrodesulfurization.

Conversion of organosulfur compounds to oxyorganosulfur compounds for

The present invention provides novel recombinant pseudomonads which contain a heterologous nucleic acid molecule comprising a nucleotide sequence encoding one or more desulfurization enzymes which are components of a biodesulfurization catalyst. The invention also provides a method of desulfurizing a carbonaceous material, such as a fossil fuel, which comprises organosulfur compounds. The method includes the steps of The method comprises the steps of (1) contacting the fossil fuel with an aqueous phase containing a recombinant biocatalyst which is capable of cleaving carbon-sulfur bonds and, optionally, a flavoprotein, thereby forming a fossil fuel and aqueous phase mixture; (2) maintaining the mixture under conditions sufficient for cleavage of the carbon-sulfur bonds of the organosulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and (3) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase.

Dsz gene expression in pseudomonas hosts

DszD utilization in desulfurization of DBT by rhodococcus sp. IGTS8

The invention relates to the discovery that the rate of reaction of the desulfurization of fossil fuels is enhanced by the addition of an oxidoreductase to the biocatalyst. The invention is drawn to a method for enhancing the rate of desulfurizing a fossil fuel containing organic sulfur compounds, comprising the steps of: a) contacting the fossil fuel with an aqueous phase containing a biocatalyst capable of cleaving carbon-sulfur bonds and a rate-enhancing amount of an oxidoreductase, thereby forming a fossil fuel and aqueous phase mixture; b) maintaining the mixture of step (a) under conditions sufficient for cleavage of the carbon-sulfur bonds of the organic sulfur molecules by the biocatalyst, thereby resulting in a fossil fuel having a reduced organic sulfur content; and c) separating the fossil fuel having a reduced organic sulfur content from the resulting aqueous phase. The invention also relates to a recombinant microorganism containing one or more recombinant DNA molecules which encode a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and which encode an oxidoreductase. The invention also relates to a composition comprising (a) a biocatalyst capable of desulfurizing a fossil fuel containing organic sulfur molecules and (b) an oxidoreductase.

DSZD utilization in desulfurization of DBT by rhodococcus sp. IGTS8

The invention relates to a method of removing metals from a fossil fuel comprising the steps of contacting the fossil fuel with a biocatalyst selected from the group consisting of an enzyme which degrades porphyrin molecules under conditions suitable for the removal of the metals from the fossil fuel; and separating the metals from the fossil fuel. Preferred embodiments of the biocatalyst include heme oxygenase and cytochrome C reductase, such as cytochrome C reductase from Bacillus megaterium, Catharanthus roseuse, Escherichia coli, animal cells, plant cells or yeast cells. The cytochrome C reductase can be contacted with the fossil fuel in an aqueous medium as a substantially cell-free preparation or cell preparation. In one embodiment of the invention, the metals are recovered from the resulting metal containing stream.

Fuel product produced by demetalizing a fossil fuel with an enzyme

The invention relates to a method of removing metals from a fossil fuel comprising the steps of contacting the fossil fuel with an oxygenase which degrades porphyrin molecules under conditions suitable for the removal of the metals from the fossil fuel; and separating the metals from the fossil fuel. Preferred embodiments of the oxygenase include heme oxygenase and cytochrome C reductase, such as cytochrome C reductase from Bacillus megaterium, Catharanthus roseuse, Escherichia coli, animal cells, plant cells or yeast cells. The cytochrome C reductase can be contacted with the fossil fuel in an aqueous medium as a substantially cell-free preparation or cell preparation. In one embodiment of the invention, the metals are recovered from the resulting metal containing stream.

Process for demetalizing a fossil fuel

A method for reducing the viscosity of viscous petroleum liquids, such as heavy crude oil and bitumen, is disclosed. The method is appropriate for use with viscous petroleum liquids that contain sulfur-bearing heterocycles, the physicochemical properties of the heterocycles contributing significantly to the viscosity of the liquid. The method comprises contacting the viscous petroleum liquid with a biocatalyst that converts sulfur-bearing heterocycles into molecules that lack physicochemical properties condusive to viscosity. The biocatalyst works in a sulfur-specific manner, such that the sulfur-bearing heterocycle is altered at the sulfur heteroatom thereof. Through biocatalysis, carbon-sulfur bonds are cleaved and/or polar substituents such as hydroxyl groups are joined to the sulfur heteroatom, the hydrocarbon framework of the sulfur-bearing heterocycle, or both. Preferred biocatalysts for viscosity reduction include preparations of Rhodococcus rhodochrous ATCC No. 53968 microorganisms and enzymes obtained therefrom.

Biocatalytic process for reduction of petroleum viscosity

The invention relates to a method of separating a multiple phase liquid medium comprising a first liquid phase and a second liquid phase wherein the medium is contacted with a first filter, said filter having been wetted by a wetting agent miscible with the first liquid phase but immiscible with the second liquid phase; whereby the first liquid phase passes through the filter, thus obtaining a filtrate substantially free of the second liquid phase. In a preferred embodiment, separation of a multiple phase liquid medium comprising liquid fossil fuel, water and biocatalyst employing one or two filters is disclosed. One filter will preferentially collect either the liquid fossil fuel or aqueous phase as the filtrate. The retentate will then flow to the second filter which will collect the component not removed before, i.e. the aqueous phase or liquid fossil fuel, as the filtrate. The remaining retentate, containing the biocatalyst, can then, preferably, be recycled. The process can be used to resolve an emulsion or microemulsion of the liquid fossil fuel and aqueous phase resulting from a BDS process. Also described is a method for controlling the reaction parameters of a mixed phase reaction process.

Method for separating a petroleum containing emulsion

A method for the deep desulfurization of a liquid fossil fuel containing organic sulfur comprising aromatic sulfur-bearing heterocycles is described, wherein the fossil fuel is (a) subjected to hydrodesulfurization or microbial desulfurization, (b) contacted with a biocatalyst in an aqueous medium in an amount and under conditions sufficient for the conversion of aromatic sulfur-bearing heterocycles to inorganic sulfur, wherein the biocatalyst comprises bacteria or a substantially cell-free preparation thereof having the capability of the parent microorganism for catalyzing the removal of sulfur from aromatic sulfur-bearing heterocycles, thereby preparing a deeply desulfurized fossil fuel; and (c) separated from the aqueous medium.

Multistage process for deep desulfurization of a fossil fuel

This invention relates to an integrated method for the desulfurization and desalting of a fossil fuel, comprising the steps of: (a) contacting a fossil fuel with; (i) a sufficient amount of an aqueous solution capable of depleting the fossil fuel of forms of water soluble salt contaminants; and (ii) an effective amount of a biocatalyst capable of depleting the fossil fuel of forms of sulfur-bearing organic molecules; (b) incubating the above mixture whereby; (i) the fossil fuel is significantly depleted of forms of water soluble salt contaminants; and (ii) the biocatalytic agent selectively catalyzes carbon-sulfur bonds in sulfur-bearing organic molecules generating a significant amount of water-soluble inorganic sulfur molecules; both reactions occurring without depleting the fossil fuel of combustible organic molecules; and (c) separating the aqueous component from the fossil fuel component, the fossil fuel now being significantly reduced in sulfur and salt contamination and the aqueous component now being significantly enriched with inorganic salts and inorganic sulfur molecules.

Growing community of inventors

The Woodlands, TX, United States of America

Daniel J Monticello